Expression and localization of maspin in cervical cancer and its role in tumor progression and lymphangiogenesis

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• 作者：Zhiqiang Liu (1)
  Yangyang Shi (2)
  Wei Meng (3)
  Yufang Liu (1)
  Kaixuan Yang (4)
  Shuhua Wu (5)
  Zhilan Peng (6)
  
• 关键词：Cervix ; CIN ; Maspin ; Lymphatic system ; Podoplanin ; Squamous cell carcinoma
• 刊名：Archives of Gynecology and Obstetrics
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：289
• 期：2
• 页码：373-382
• 全文大小：625 KB
• 作者单位：Zhiqiang Liu (1)
  Yangyang Shi (2)
  Wei Meng (3)
  Yufang Liu (1)
  Kaixuan Yang (4)
  Shuhua Wu (5)
  Zhilan Peng (6)
  
  1. Department of Obstetrics and Gynecology, Affiliated Hospital of Binzhou Medical College, Binzhou, 256603, China
  2. Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing, 100034, China
  3. Laboratory of Pathogenic Organism, Affiliated Hospital of Binzhou Medical College, Binzhou, 256603, China
  4. Department of Pathology, West China Second Hospital (Women and Infants Hospital) of Sichuan University, Chengdu, Sichuan, 610041, China
  5. Department of Pathology, Affiliated Hospital of Binzhou Medical College, Binzhou, 256603, China
  6. Department of Obstetrics and Gynecology, West China Second Hospital (Women and Infants Hospital) of Sichuan University, No.17, Renmin Road South, Chengdu, Sichuan, 610041, China
  
• ISSN：1432-0711

文摘

Objectives Cervical cancer is the most common malignant tumor in female reproductive tract and primarily metastasizes through the lymphatic system that will affect prognosis of patients. Maspin, a member of the serine protease inhibitors (serpins) super family, has recently been indicated as a tumor suppressor in many cancers. In this study, we investigated the clinical significance of maspin expression, especially the subcellular location of maspin and its functional role in progression and lymphangiogenesis, in cervical squamous cell carcinoma. Methods Labelled streptavidin biotin method (LSAB) was used to determine cytoplasmic and nuclear maspin expressions, respectively, in 13 cases of normal cervix, 15 cases of cervical intraepithelial neoplasia grade 3 (CIN3), 62 cases of squamous cell carcinoma (SCC) of the uterine cervix, and 13 cases of pelvic lymphatic nodes which were all positive lymphatic nodes in our selected cancer cases. LSAB is also used to detect podoplanin which is used for counting density of lymphatic microvessels (LMVD). The clinical significance of subcellular maspin expression and the relationship between maspin expression and LMVD in cervical cancer are analyzed. Results Both cytoplasmic and nuclear maspin expressions in SCC were significantly weaker than those of normal cervix and CIN3. Nuclear maspin expression showed a peak in CIN3 and then dropped in SCC. Declined maspin expression was correlated with later clinical stage, increased LMVD, and lymphatic metastasis. Conclusions Our results suggest that subcellular location of maspin expression is a potential predictive factor in tumor progression and in patients-prognosis of cervical cancer, and maspin plays a suppression role in lymphangiogenesis and metastasis.