Ultra-precise detection of mutations by droplet-based amplification of circularized DNA
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- 作者：Kaile Wang ; Qin Ma ; Lan Jiang ; Shujuan Lai ; Xuemei Lu ; Yali Hou ; Chung-I Wu…
- 关键词：Accurate sequencing ; Low frequency mutation detection ; Low bias amplification ; Droplet (water in oil) based amplification ; Low input NGS library
- 刊名：BMC Genomics
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：17
- 期：1
- 全文大小：2,613 KB
- 刊物主题：Life Sciences, general; Microarrays; Proteomics; Animal Genetics and Genomics; Microbial Genetics and Genomics; Plant Genetics & Genomics;
- 出版者：BioMed Central
- ISSN：1471-2164
文摘
Background NGS (next generation sequencing) has been widely used in studies of biological processes, ranging from microbial evolution to cancer genomics. However, the error rate of NGS (0.1 % ~ 1 %) is still remaining a great challenge for comprehensively investigating the low frequency variations, and the current solution methods have suffered severe amplification bias or low efficiency.