Sulfated Polysaccharide Isolated from the Sea Cucumber Stichopus japonicus Against PC12 Hypoxia/Reoxygenation Injury by Inhibition of the MAPK Signaling Pathway

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• 作者：Chao Cui ; Ningshan Cui ; Peng Wang ; Shuliang Song…
• 关键词：Hypoxia/reoxygenation(H/R) ; PC12 ; Na2S2O4 ; MAPK
• 刊名：Cellular and Molecular Neurobiology
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：35
• 期：8
• 页码：1081-1092
• 全文大小：1,997 KB
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• 作者单位：Chao Cui (1) (2)
  Ningshan Cui (1)
  Peng Wang (2)
  Shuliang Song (1)
  Hao Liang (1)
  Aiguo Ji (1) (2)
  
  1. Marine College, Shandong University, Weihai, Shandong, China
  2. School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Neurosciences
  Animal Anatomy, Morphology and Histology
  
• 出版者：Springer Netherlands
• ISSN：1573-6830

文摘

In this report, the sulfated polysaccharide (SJP) isolated from the sea cucumber Stichopus japonicus can protect PC12 from Na₂S₂O₄-induced hypoxia/reoxygenation (H/R) injury. SJP effectively improves cell viability and reduces extracellular LDH release in PC12 cells after H/R. Moreover, SJP significantly increases SOD activity but decreases MDA levels. Our experiments showed that SJP could significantly reduce cell apoptosis caused by H/R. Our current results demonstrate that SJP suppressed the activation of MAPKs, resulting in a significant decrease in Bax/Bcl-2 ratio, cleaved caspase-3/caspase-3, p53 phosphorylation, and cytochrome c release in a concentration-dependent manner. MAPK is closely related to H/R injury. SJP inhibited JNK1/2 and p38 MAPK activation but did not affect the increased ERK1/2 expression. These results suggested that JNK1/2 and p38 MAPK pathways could be involved in SJP-mediated attenuation of PC12 H/R injury. SJP prevented PC12 H/R injury in a dose-dependent manner, indicating that SJP may be developed as a candidate drug to prevent or treat cerebral ischemia–reperfusion injury. Keywords Hypoxia/reoxygenation(H/R) PC12 Na₂S₂O₄ MAPK