Comparative study between macrolide regulatory proteins MphR(A) and MphR(E) in ligand identification and DNA binding based on the rapid in vitro detection system

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• 作者：Yongyou Cheng ; Shuming Yang ; Man Jia ; Luyao Zhao…
• 关键词：Macrolide antibiotic ; Macrolide regulatory protein MphR(A) ; Macrolide regulatory protein MphR(E) ; Rapid in vitro detection ; ELISA ; type system ; Protein ; DNA binding ; dissociation
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：408
• 期：6
• 页码：1623-1631
• 全文大小：833 KB
• 参考文献：1.Weisblum B. Erythromycin resistance by ribosome modification. Antimicrob Agents Chemother. 1995;39:577–85.CrossRef
  2.Ross JI, Eady EA, Cove JH, Cunliffe WJ, Baumberg S, Wootton JC. Inducible erythromycin resistance in staphylococci is encoded by a member of the ATP-binding transport super-gene family. Mol Microbiol. 1990;4:1207–14.CrossRef
  3.Leclercq R, Courvalin P. Intrinsic and unusual resistance to macrolide, lincosamide, and streptogramin antibiotics in bacteria. Antimicrob Agents Chemother. 1991;35:1273–6.CrossRef
  4.Noguchi N, Emura A, Matsuyama H, O’Hara K, Sasatsu M, Kono M. Nucleotide sequence and characterization of erythromycin resistance determinant that encodes macrolide 2′-phosphotransferase I in Escherichia coli. Antimicrob Agents Chemother. 1995;39:2359–63.CrossRef
  5.Noguchi N, Takada K, Katayama J, Emura A, Sasatsu M. Regulation of transcription of the mph(A) gene for macrolide 2'-phosphotransferase I in Escherichia coli: characterization of the regulatory gene mphR(A). J Bacteriol. 2000;182:5052–8.CrossRef
  6.Noguchi N, Katayama J. Expression in Pseudomonas aeruginosa of an erythromycin-resistance determinant that encodes the mphA gene for macrolide 20-phosphotransferase I from Escherichia coli. Biol Pharm Bull. 1998;21:191–3.CrossRef
  7.Noguchi N, Katayama J, Sasatsu M. A transposon carrying the gene mphB for macrolide 20-phosphotransferase II. FEMS Microbiol Lett. 2000;192(2):175–8.
  8.Poole TL, Callaway TR, Bischoff KM, Warnes CE, Nisbet DJ. Macrolide inactivation gene cluster mphA-mrx-mphR adjacent to a class 1 integron in Aeromonas hydrophila isolated from a diarrhoeic pig in Oklahoma. J Antimicrob Chemother. 2006;57:31–8.CrossRef
  9.Szczepanowski R, Braun S, Riedel V, Schneiker S, Krahn I, Pühler A, et al. The 120 592 bp IncF plasmid pRSB107 isolated from a sewage-treatment plant encodes nine different antibiotic-resistance determinants, two iron-acquisition systems and other putative virulence-associated functions. Microbiology. 2005;151:1095–111.CrossRef
  10.Szczepanowski R, Krahn I, Linke B, Goesmann A, Pühler A, Schlüter A. Antibiotic multiresistance plasmid pRSB101 isolated from a wastewater treatment plant is related to plasmids residing in phytopathogenic bacteria and carries eight different resistance determinants including a multidrug transport system. Microbiology. 2004;150:3613–30.CrossRef
  11.Ramos JL, Martínez-Bueno M, Molina-Henares AJ, Terán W, Watanabe K, Zhang X, et al. The TetR family of transcriptional repressors. Microbiol Mol Biol Rev. 2005;69:326–56.CrossRef
  12.Zheng J, Sagar V, Smolinsky A, Bourke C, LaRonde-LeBlanc N, Cropp TA. Structure and function of the macrolide biosensor protein, MphR(A), with and without erythromycin. J Mol Biol. 2009;387:1250–60.CrossRef
  13.Weber W, Fux C, Daoud-el Baba M, Keller B, Weber CC, Kramer BP, et al. Macrolide-based transgene control in mammalian cells and mice. Nat Biotechnol. 2002;20:901–7.CrossRef
  14.Link N, Weber W, Fussenegger M. A novel generic dipstick-based technology for rapid and precise detection of tetracycline, streptogramin and macrolide antibiotics in food samples. J Biotechnol. 2007;128:668–80.CrossRef
  15.Weber CC, Link N, Fux C, Zisch AH, Weber W, Fussenegger M. Broad-spectrum protein biosensors for class-specific detection of antibiotics. Biotechnol Bioeng. 2005;89(1):9–17.CrossRef
  16.Möhrle V, Stadler M, Eberz G. Biosensor-guided screening for macrolides. Anal Bioanal Chem. 2007;388:1117–25.CrossRef
  17.Szczepanowski R, Linke B, Krahn I, Gartemann KH, Gützkow T, Eichler W, et al. Detection of 140 clinically relevant antibiotic-resistance genes in the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to selected antibiotics. Microbiology. 2009;155:2306–19.CrossRef
  18.Szczepanowski R, Krahn I, Bohn N, Pühler A, Schlüter A. Novel macrolide resistance module carried by the IncP-1β resistance plasmid pRSB111, isolated from a wastewater treatment plant. Antimicrob Agents Chemother. 2007;51:673–8.CrossRef
  19.Orth P, Schnappinger D, Hillen W, Saenger W, Hinrichs W. Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system. Nat Struct Biol. 2000;7:215–9.CrossRef
  20.Grkovic S, Brown MH, Roberts NJ, Paulsen IT, Skurray RA. QacR is a repressor protein that regulates expression of the Staphylococcus aureus multidrug efflux pump QacA. J Biol Chem. 1998;273:18665–73.CrossRef
  21.Grkovic S, Brown MH, Schumacher MA, Brennan RG, Skurray RA. Staphylococcal QacR multidrug regulator binds a correctly spaced operator as a pair of dimers. J Bacteriol. 2001;183:7102–9.CrossRef
  22.Schumacher MA, Miller MC, Grkovic S, Brown MH, Skurray RA, Brennan RG. Structural basis for cooperative DNA binding by two dimers of the multidrug-binding protein QacR. EMBO J. 2002;21:1210–8.CrossRef
  23.Virolainen N. Antimicrobial detection illuminated: developing bioluminescent antibiotic biosensors based on bacterial gene regulatory elements. Tampere: Tampere University of Technology; 2012.
  24.Kamionka A, Majewski M, Roth K, Bertram R, Kraft C, Hillen W. Induction of single chain tetracycline repressor requires the binding of two inducers. Nucleic Acids Res. 2006;34(14):3834–41.CrossRef
  25.Viitamaki HJ. Towards a macrolide biosensor: mutation and production of MphR(E) repressor protein. Tampere: University of Tampere, Institute of Biomedical Technology; 2011.
  
• 作者单位：Yongyou Cheng (1)
  Shuming Yang (1)
  Man Jia (1)
  Luyao Zhao (1)
  Can Hou (1)
  Xinyong You (1)
  Jie Zhao (1)
  Ailiang Chen (1)
  
  1. Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences (CAAS), No. 12 Zhongguancun South St., Haidian District, Beijing, 100084, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

The macrolide regulatory protein MphR(A) has been widely studied and used in various aspects such as metabolism monitoring, exogenous gene expression, and in vivo and in vitro macrolide antibiotic screening. Another macrolide regulatory protein, MphR(E), has rarely been reported. In this study, in vitro ELISA-type systems were established for MphR(A) and MphR(E) to study their correlation. The reactivity of 14 macrolide antibiotics and pseudo-macrolide antibiotics was tested in the systems. The results indicated that the ligand identification spectra of MphR(A) and MphR(E) were basically consistent. The binding characteristics of MphR(A) and MphR(E) with three corresponding promoter DNA sequences were preliminarily studied. According to the ELISA-type analysis results, MphR(A) and MphR(E) have consistent DNA binding properties, which bind to A-DNA/B-DNA more easily than to C-DNA. This study has confirmed that MphR(E) can bind to the promoter DNA sequences mrx(E) and mph(E) in plasmid pRSB111, and different DNAs can affect the sensitivity of the in vitro detection systems.