Imbalance between proliferation and apoptosis−related impaired GPR30 expression is involved in preeclampsia

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• 作者：Jianxin Li ; Zhu Chen ; Xiaobo Zhou ; Shuming Shi ; Hongbo Qi…
• 关键词：GPR30 ; Preeclampsia ; Proliferation ; Apoptosis ; Placenta
• 刊名：Cell and Tissue Research
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：366
• 期：2
• 页码：499-508
• 全文大小：11,864 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Human Genetics
  Proteomics
  Molecular Medicine
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0878
• 卷排序：366

文摘

The proliferation and apoptosis of cells in the placenta play a critical role in preeclampsia (PE) in which estrogen has been implicated via estrogen receptors (ERs). A novel ER, G-protein-coupled receptor 30 (GPR30), has recently been shown to be involved in PE. We investigated the basic levels of proliferation and apoptosis in normal placentae and placentae with PE and compared GPR30 expression levels between the two groups. We demonstrated that low GPR30 expression levels, more apoptosis, and less proliferation were associated with PE. Moreover, our in vitro study showed that both the selective GPR30 agonist G1 and the general ER agonist 17-β-estradiol were able to protect the placenta from hypoxia-reoxygenation injuries, resulting in decreased apoptosis and increased proliferation. Furthermore, this protective effect was abolished by the addition of the selective GPR30 inhibitor G15. These results provide evidence that (1) GPR30 is involved in regulating cell proliferation and apoptosis; (2) pharmacologic upregulation of GPR30 is beneficial for PE management; (3) GPR30 may therefore be an interventional target for pregnancies complicated by PE.