Delta-catenin promotes the proliferation and invasion of colorectal cancer cells by binding to E-cadherin in a competitive manner with p120 catenin

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• 作者：Hong Zhang (1)
  Shun-Dong Dai (2) (3)
  Di Zhang (2) (3)
  Dong Liu (1)
  Fang-Yuan Zhang (1)
  Tian-Yi Zheng (1)
  Ming-Ming Cui (1)
  Chao-Liu Dai (1)
  
• 关键词：Colorectal cancer ; Delta ; catenin ; E ; cadherin ; p120 catenin ; Invasion ; Metastasis
• 刊名：Targeted Oncology
• 出版年：2014
• 出版时间：March 2014
• 年：2014
• 卷：9
• 期：1
• 页码：53-61
• 全文大小：1,232 KB
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• 作者单位：Hong Zhang (1)
  Shun-Dong Dai (2) (3)
  Di Zhang (2) (3)
  Dong Liu (1)
  Fang-Yuan Zhang (1)
  Tian-Yi Zheng (1)
  Ming-Ming Cui (1)
  Chao-Liu Dai (1)
  
  1. Department of Colorectal Surgery, Shengjing Hospital, China Medical University, Shenyang, Liaoning, China
  2. Department of Pathology, the First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, Liaoning, China
  3. Institute of Pathology and Pathophysiology, China Medical University, Shenyang, Liaoning, China
  
• ISSN：1776-260X

文摘

δ-Catenin is the only member of the p120 catenin (p120ctn) subfamily whose normal pattern of expression is restricted to the brain. Similar to p120ctn, δ-catenin can bind to the juxtamembrane domain of E-cadherin. We examined the expression of δ-catenin, p120ctn, and E-cadherin using immunohistochemistry in 95 cases of colorectal cancer (CRC) and 15 normal colon tissues. Co-immunoprecipitation was used to examine whether δ-catenin competed with p120ctn to bind E-cadherin in CRC cells. The effects of δ-catenin overexpression or siRNA-mediated knockdown on the proliferation and invasive ability of CRC cells were investigated using the MTT and Matrigel invasion assays. The results showed that positive δ-catenin expression was significantly more frequent in CRC compared to normal colon tissues and associated with poor differentiation, stage III–IV disease, and lymph node metastasis in CRC (all P-lt;-.05). In two CRC cell lines, δ-catenin bound to E-cadherin in competition with p120ctn. Overexpression of δ-catenin promoted the proliferation and invasion of CRC cells; knockdown of δ-catenin reduced CRC cell proliferation and invasion. In conclusion, we speculate that overexpression of δ-catenin reduces the expression of E-cadherin and alters the balance between E-cadherin and p120ctn, which in turn affects the formation of intercellular adhesions and promotes invasion and metastasis in CRC.