Xiao-Qing-Long-Tang shows preventive effect of asthma in an allergic asthma mouse model through neurotrophin regulation

详细信息    查看全文

• 作者：Ren-Shiu Chang (11) (11)
  Shulhn-Der Wang (7) (8)
  Yu-Chin Wang (10)
  Li-Jen Lin (8)
  Shung-Te Kao (8) (9)
  Jiu-Yao Wang (10)
  
• 关键词：Asthma ; Xiao ; Qing ; Long ; Tang (XQLT) ; Nerve growth factor (NGF) ; Brain ; derived neurotrophic factor (BDNF) ; p75 neurotrophin receptor (p75NTR)
• 刊名：BMC Complementary and Alternative Medicine
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：13
• 期：1
• 全文大小：1644KB
• 参考文献：1. Kung YY, Chen YC, Hwang SJ, Chen TJ, Chen FP, / et al.: The prescriptions frequencies and patterns of Chinese herbal medicine for allergic rhinitis in Taiwan. / Allergy 2006, 61:1316-318. CrossRef
  2. Nagai T, Nakao M, Shimizu Y, Kodera Y, Oh-Ish M, Maeda T, / et al.: Proteomic analysis of anti-inflammatory effects of a kampo (japanese herbal) medicine “Shoseiryuto (Xiao-Qing-Long-Tang)-on airway inflammation in a mouse model. / Evid Based Complement Alternat Med 2011, 2011:1-3. CrossRef
  3. Kao ST, Wang SD, Wang JY, Yu CK, Lei HY, / et al.: The effect of Chinese herbal medicine, xiao-qing-long tang (XQLT), on allergen-induced bronchial inflammation in mite-sensitized mice. / Allergy 2000, 55:1127-133. CrossRef
  4. Nagai T, Arai Y: Anti-allergic activity of a Kampo (Japanese herbal) medicine “Sho-seiryu-to (Xiao-Qing-Long-Tang)-on airway inflammation in a mouse model. / Int Immunopharmacol 2004, 4:1353-365. CrossRef
  5. Eunjung KO, Samwoong RHO: So-Cheong-Ryong-Tang, tradititional Korean medicine, suppresses Th2 lineage development. / Biol Pharm Bull 2004,27(5):739-43. CrossRef
  6. Ko E, Rho S, Lee EJ, Seo YH, Cho C, Lee Y: Traditional Korean medicine (SCRT) modulate Th1/Th2 specific cytokine production in mice CD4+ T cell. / J Ethnopharmacol 2004, 92:121-28. CrossRef
  7. Hoyle GW, Graham RM: Hyperinnervation of the airways in transgenic mice overexpressing nerve growth factor. / Am J Respir Cell Mol Bio1 1998,18(2):149-57. CrossRef
  8. Ehrhard PB, Erb P, Graumann U, Otten U, / et al.: Expression of nerve growth factor and nerve growth factor receptor tyrosine kinase Trk in activated CD4-positive T-cell clones. / Proc Natl Acad Sci U S A 1993, 90:10984-0988. CrossRef
  9. Ehrhard PB, Erb P, Graumann U, Schmutz B, Otten U: Expression of functional trk tyrosine kinase receptors after T cell activation. / J Immunol 1994, 152:2705-709.
  10. Ye YL, Wu HT, Lin CF, / et al.: Dermatophagoides pteronyssinus 2 regulates nerve growth factor release to induce airway inflammation via a reactive oxygen species-dependent pathway. / Am J Physiol Lung Cell Mol Physiol 2011, 300:L216-L224. CrossRef
  11. Nassenstein C, Braun A: The neurotrophins nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3, and neurotrophin-4 are survival and activation factors for eosinophils in patients with allergic bronchial asthma. / J Exp Med 2003,198(3):455-67. CrossRef
  12. Hahn C, Islamian AP, Renz H, Nockher WA: Airway epithelial cells produce neurotrophins and promote the survival of eosinophils during allergic airway inflammation. / J Allergy Clin Immunol 2006,117(4):787-94. CrossRef
  13. Noga O, Peiser M, Alten?hr M, / et al.: Differential activation of dendritic cells by nerve growth factor and brain-derived neurotrophic factor. / Clin Exp Allergy 2007,37(11):1701-708. CrossRef
  14. Lommatzsch M, Braun A: Neurotrophins in allergic airway dysfunction: what the mouse model is teaching us. / Ann N Y Acad Sci 2003, 992:241-49. CrossRef
  15. Dagnell C, Kemi C: Effects of neurotrophins on human bronchial smooth muscle cell migration and matrix metalloproteinase-9 secretion. / Transl Res 2007, 150:303-10. CrossRef
  16. Aravamudan B, Thompson M: Brain derived neurotrophic factor induces proliferation of human airway smooth muscle cells. / J Cell Mol Med 2012,86(4):812-23. CrossRef
  17. Martinowich K, Manji H, Lu B: New insights into BDNF function in depression and anxiety. / Nat Neurosci 2007,10(9):1089-093. CrossRef
  18. McGurk JS, Shim S, Kim JY, Wen Z, Song H, Ming GL: Postsynaptic TRPC1 function contributes to BDNF-induced synaptic potentiation at the developing neuromuscular junction. / J Neurosci 2012,31(41):14754-4762. CrossRef
  19. Tokuoka S, Takahashi Y, Masuda T, Tanaka H, Furukawa S, Nagai H: Disruption of antigen-induced airway inflammation and airway hyper-responsiveness in low affinity neurotrophin receptor p75 gene deficient mice. / Br J Pharmacol 2001, 134:1580-586. CrossRef
  20. Kerzel S, P?th G, Nockher WA, / et al.: Pan-neurotrophin receptor p75 contributes to neuronal hyperreactivity and airway inflammation in a murine model of experimental asthma. / Am J Respir Cell Mol Biol 2003,28(2):170-78. CrossRef
  21. Bothwell M: p75NTR: a receptor after all. / Science 1996, 272:506-07. CrossRef
  22. Carter BD, Kaltschmidt C: Selective activation of NF-kappa B by nerve growth factor through the neurotrophin receptor p75. / Science 1996, 272:542-45. CrossRef
  23. Jiang Y, Chen G: Nerve growth factor promotes TLR4 signaling-induced maturation of human dendritic cells in vitro through inducible p75NTR. / J Immuno 2007, 179:6297-304.
  24. Wang SD, Lin LJ, Chen CL, Lee SC, Lin CC, Wang JY, Kao ST: Xiao-Qing-Long-Tang attenuates allergic airway inflammation and remodeling in repetitive Dermatogoides pteronyssinus challenged chronic asthmatic mice model. / J Ethnopharmacol 2012,142(2):531-38. CrossRef
  25. Vanoirbeek JA, Rinaldi M, De Vooght V, / et al.: Noninvasive and invasive pulmonary function in mouse models of obstructive and restrictive respiratory diseases. / Am J Respir Cell Mol Biol 2010,42(1):96-04. CrossRef
  26. De Vleeschauwer SI, Rinaldi M, De Vooght V, / et al.: Repeated invasive lung function measurements in intubated mice: an approach for longitudinal lung research. / Lab Anim 2011,45(2):81-9. CrossRef
  27. Glaab T, Hoymann HG, Hecht M, / et al.: Effect of anti-nerve growth factor on early and late airway responses in allergic rats. / Allergy 2003, 58:900-04. CrossRef
  28. Lee HW, Na YJ, Jung KP: Nerve growth factor stimulates proliferation, adhesion and thymopoietic cytokine expression in mouse thymic epithelial cells in vitro. / Regul Pept 2011, 147:72-1. CrossRef
  29. Kato T, Saeki H, Tsunemi Y, Shibata S, Tamaki K, Sato S: Thymus and activation-regulated chemokine (TARC)?CC chemokine ligand (CCL) 17 accelerates wound healing by enhancing fibroblast migration. / Exp Dermatol 2011,20(8):669-74. CrossRef
  30. Arazi A, Sharabi A, Zinger H, Mozes E, Neumann AU: In vivo dynamical interactions between CD4 Tregs, CD8 Tregs and CD4+ CD25- cells in mice. / PLoS One 2009,4(12):e8447. CrossRef
  31. Noble A, Giorgini A, Leggat JA: Cytokine-induced IL-10-secreting CD8 T cells represent a phenotypically distinct suppressor T-cell lineage. / Blood 2006,107(11):4475-483. CrossRef
  32. García-Suárez O, Pérez-Pinera P, Laurà R, / et al.: TrkB is necessary for the normal development of the lung. / Respir Physiol Neurobiol 2009,167(3):281-91. CrossRef
  33. Wong I, Liao H, Bai X, Zaknic A, / et al.: ProBDNF inhibits infiltration of ED1+ macrophages after spinal cord injury. / Brain Behav Immun 2010,24(4):585-97. CrossRef
  34. Miyazaki E, Nureki SI, Fukami T, Shigenaga T, / et al.: Elevated levels of thymus- and activation-regulated chemokine in bronchoalveolar lavage fluid from patients with eosinophilic pneumonia. / Am J Respir Crit Care Med 2002, 165:1125-131. CrossRef
  35. Thielen C, Radermacher V, Trimeche M, / et al.: TARC and IL-5 expression correlates with tissue eosinophilia in peripheral T-cell lymphomas. / Leuk Res 2008,32(9):1431-438. CrossRef
  36. Berin MC: The role of TARC in the pathogenesis of allergic asthma. / Drug News Perspect 2002,15(1):10-6. CrossRef
  37. Lee MY, Shin IS, Jeon WY, Lim HS, Kim JH, Ha H: Pinellia ternata Breitenbach attenuates ovalbumin-induced allergic airway inflammation and mucus secretion in a murine model of asthma. / Immunopharmacol Immunotoxicol 2013,35(3):410-18. CrossRef
  38. Shin IS, Lee MY, Jeon WY, Shin NR, Seo CS, Ha H: EBM84 attenuates airway inflammation and mucus hypersecretion in an ovalbumin-induced murine model of asthma. / Int J Mol Med 2013,31(4):982-88.
  39. The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/13/220/prepub
  
• 作者单位：Ren-Shiu Chang (11) (11)
  Shulhn-Der Wang (7) (8)
  Yu-Chin Wang (10)
  Li-Jen Lin (8)
  Shung-Te Kao (8) (9)
  Jiu-Yao Wang (10)
  
  11. Department of Chinese Medicine, Tainan Sin-Lau Hospital, No. 57, Sec. 1, Dongmen Rd, Tainan, 70142, Taiwan
  7. School of Post-Baccalaureate Chinese Medicine, College of Chinese Medicine, China Medical University, No. 91 Hsueh-Shih Road, Taichung, 40402, Taiwan
  8. School of Chinese Medicine, College of Chinese Medicine, China Medical University, No. 91 Hsueh-Shih Road, Taichung, 40402, Taiwan
  10. Department of Pediatrics, College of Medicine, National Cheng Kung University, No. 138, Sheng-Li Road, Tainan, 70428, Taiwan
  9. Department of Chinese Medicine, China Medical University Hospital, No. 2 Yude Road, Taichung, 40447, Taiwan
  

文摘

Background This study investigates the effect of Xiao-Qing-Long-Tang (XQLT) on neurotrophin in an established mouse model of Dermatophagoides pteronyssinus (Der p)-induced acute allergic asthma and in a LA4 cell line model of lung adenoma. The effects of XQLT on the regulation of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF), airway hyper-responsiveness (AHR) and immunoglobulin E were measured. Methods LA4 cells were stimulated with 100 μg/ml Der p 24 h and the supernatant was collected for ELISA analysis. Der p-stimulated LA4 cells with either XQLT pre-treatment or XQLT co-treatment were used to evaluate the XQLT effect on neurotrophin. Balb/c mice were sensitized on days 0 and 7 with a base-tail injection of 50 μg Dermatophagoides pteronyssinus (Der p) that was emulsified in 50 μl incomplete Freund’s adjuvant (IFA). On day 14, mice received an intra-tracheal challenge of 50 μl Der p (2 mg/ml). XQLT (1g/Kg) was administered orally to mice either on days 2, 4, 6, 8, 10 and 12 as a preventive strategy or on day 15 as a therapeutic strategy. Results XQLT inhibited expression of those NGF, BDNF and thymus-and activation-regulated cytokine (TARC) in LA4 cells that were subjected to a Der p allergen. Both preventive and therapeutic treatments with XQLT in mice reduced AHR. Preventive treatment with XQLT markedly decreased NGF in broncho-alveolar lavage fluids (BALF) and BDNF in serum, whereas therapeutic treatment reduced only serum BDNF level. The reduced NGF levels corresponded to a decrease in AHR by XQLT treatment. Reduced BALF NGF and TARC and serum BDNF levels may have been responsible for decreased eosinophil infiltration into lung tissue. Immunohistochemistry showed that p75NTR and TrkA levels were reduced in the lungs of mice under both XQLT treatment protocols, and this reduction may have been correlated with the prevention of the asthmatic reaction by XQLT. Conclusion XQLT alleviated allergic inflammation including AHR, IgE elevation and eosinophil infiltration in Der p stimulated mice by regulating neurotrophin and reducing TARC. These results revealed the potential pharmacological targets on which the XQLT decotion exerts preventive and therapeutic effects in an allergic asthma mouse model.