A mediator-free amperometric hydrogen peroxide biosensor based on HRP immobilized on a nano-Au/poly 2,6-pyridinediamine-coated electrode

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• 作者：Shurui Cao ; Ruo Yuan ; Yaqin Chai ; Lingyan Zhang ; Xuelian Li and Fengxian Gao
• 关键词：2 ; 6 ; Pyridinediamine ; Colloidal Au ; Horseradish peroxidase ; Biosensor ; Electropolymerization
• 刊名：Bioprocess and Biosystems Engineering
• 出版年：2007
• 出版时间：March, 2007
• 年：2007
• 卷：30
• 期：2
• 页码：71-78
• 全文大小：221 KB

文摘

A mediator-free amperometric hydrogen peroxide biosensor was prepared by immobilizing horseradish peroxidase (HRP) enzyme on colloidal Au modified platinum (Pt) wire electrode, which was modified by poly 2,6-pyridinediamine (pPA). The modified process was characterized by electrochemical impedance spectroscopy (EIS), and the electrochemical characteristics of the biosensor were studied by cyclic voltammetry, linear sweep voltammetry and chronoamperometry. The biosensor displayed an excellent electrocatalytical response to reduction of H₂O₂ without the aid of an electron mediator, the linear range was 4.2 ¡Á 10−7–1.5 ¡Á 10−3 mol/L (r = 0.9977), with a detection limit of 1.4 ¡Á 10−7 mol/L. Moreover, the performance and factors influencing the resulted biosensor were studied in detail. The studied biosensor exhibited permselectivity, good stability and good fabrication reproducibility.