Amperometric glucose biosensors based on layer-by-layer assembly of chitosan and glucose oxidase on the Prussian blue-modified gold electrode

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• 作者：Bing Yin ; Ruo Yuan ; Yaqing Chai ; Shihong Chen ; Shurui Cao ; Yang Xu and Ping Fu
• 关键词：Chitosan ; Glucose biosensor ; Glucose oxidase ; Layer ; by ; layer assembly ; Prussian blue
• 刊名：Biotechnology Letters
• 出版年：2008
• 出版时间：February, 2008
• 年：2008
• 卷：30
• 期：2
• 页码：317-322
• 全文大小：563.9 KB

文摘

A glucose biosensor based on layer-by-layer (LBL) self-assembling of chitosan and glucose oxidase (GOD) on a Prussian blue film was developed. First, Prussian blue was deposited on a cleaned gold electrode then chitosan and GOD were assembled alternately to construct a multilayer film. The resulting amperometric glucose biosensor exhibited a fast response time (within 10 s) and a linear calibration range from 6 μM to 1.6 mM with a detection limit of 3.1 μM glucose (s/n = 3). With the low operating potential, the biosensor showed little interference to the possible interferents, including ascorbic acid, acetaminophen and uric acid, indicating an excellent selectivity.