A Specific and High-Throughput Fluorescence Polarization Immunoassay for Surveillance Screening of Clinafloxacin in Milk
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- 作者：Jiahong Chen ; Shuwei Lv ; Qiang Wang ; Zhenlin Xu ; Jinyi Yang…
- 关键词：Clinafloxacin ; Homogeneous ; Fluorescence polarization immunoassay ; Milk
- 刊名：Food Analytical Methods
- 出版年：2015
- 出版时间：July 2015
- 年：2015
- 卷：8
- 期：6
- 页码：1468-1476
- 全文大小：922 KB
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Shuwei Lv (1)
Qiang Wang (2)
Zhenlin Xu (1)
Jinyi Yang (1)
Yudong Shen (1)
Hong Wang (1)
Yuanming Sun (1)
Hongtao Lei (1)
1. Laboratory of Quality & Safety Risk Assessment for Agro-Products on Storage and Preservation (Guangzhou), Ministry of Agriculture/Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou, 510642, China
2. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China - 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Food Science
Chemistry
Microbiology
Analytical Chemistry - 出版者：Springer New York
- ISSN：1936-976X
文摘
In this study, antibody specific to clinafloxacin (CLI) was generated for the first time, and a rapid and specific surveillance screening method, fluorescence polarization immunoassay (FPIA), was developed for the detection of CLI residue in milk. The developed FPIA showed a working range from 0.8 to 787.3?μg/L with the IC50 of 25.6?μg/L. The cross-reactivity to other fluoroquinolone drugs was negligible, which demonstrated high specificity. Good recoveries, ranged from 97.9 to 112.7?%, in spiked milk samples were obtained. The proposed FPIA was also validated by high performance liquid chromatography as a reference method, and the two methods yielded concordant results. The investigation indicated that the proposed FPIA is suitable for surveillance screening purposes to detect CLI residue in milk.