Inhibitory Effect of α-Tocopherol on Benzo(a)pyrene-Induced CYPA1 Activity in Rat Liver
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- 作者：Yu. A. Sidorova and A. Yu. Grishanova
- 关键词：α ; tocopherol ; benzo(a)pyrene ; CYP1A ; NADPH ; cytochrome P450 reductase ; glutathione S ; transferase
- 刊名：Bulletin of Experimental Biology and Medicine
- 出版年：2005
- 出版时间：November 2005
- 年：2005
- 卷：140
- 期：5
- 页码：517-520
- 全文大小：279 KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Biomedicine
Biomedicine
Internal Medicine
Cell Biology
Pathology
Laboratory Medicine
Russian Library of Science - 出版者：Springer New York
- ISSN：1573-8221
文摘
Combined treatment with benzo(a)pyrene (classic inductor of cytochromes P450 of subfamily 1A, CYP1A1 and CYP1A2) and α-tocopherol decreased benzo(a)pyrene-induced CYP1A1 activity in rat liver. Activities of CYP1A2, NADPH-cytochrome P450 reductase, and glutathione S-transferase remained unchanged under these conditions. Addition of α-tocopherol to benzo(a)pyrene-induced microsomes in vitro decreased activity of CYP1A1. Immunoblotting of proteins in liver microsomes with antibodies against CYP1A1 did not reveal differences in CYP1A1 protein content in the liver of rats receiving benzo(a)pyrene alone or in combination with α-tocopherol. The in vivo decrease in benzo(a)pyrene-induced CYP1A1 activity did not result from free radical-produced damage to CYP1A1. The inhibition of benzo(a)pyrene-induced CYP1A1 activity with α-tocopherol is probably realized at the posttranslational level.