Expression and antigen activity determination of human thyroid peroxidase in silkworm and yeast

详细信息    查看全文

• 作者：Baodang Guo ; Wanli Gao ; Huifang Lü…
• 关键词：Key wordshuman thyroid peroxidase ; silkworm larvae ; Pichia pastoris ; antigen activity ; cost effective
• 刊名：Wuhan University Journal of Natural Sciences
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：21
• 期：6
• 页码：531-536
• 全文大小：506 KB
• 刊物类别：Mathematics and Statistics
• 刊物主题：Mathematics
  Computer Science, general
  Physics
  Life Sciences
  Chinese Library of Science
  
• 出版者：Wuhan University, co-published with Springer
• ISSN：1993-4998
• 卷排序：21

文摘

In this study, we report the expression of human thyroid peroxidase (TPO) in silkworm larvae and Pichia pastoris GS115. Recombinant TPO is sequentially purified from the hemolymph of infected silkworm larvae and yeast using a Ni-NTA resin kit. The concentration of yield of recombinant TPO is 4.87 mg per thousand larvae and 40.83 mg per liter yeast culture. However, the recombinant TPO produced in silkworm show similar binding ability with the specific anti-TPO serum to standard human TPO purified from insect cells. The lower antigen activity indicates the TPO expressed in yeast is not suitable to be used as the coating antigen in enzyme linked immunosorbent assay (ELISA). The cost of TPO expressed in B. mori is about 1/4 that of in insect cells, and the cost of TPO purified from silkworm for ELISA is only 1/8 that of TPO produced from Sf9 cells. It indicates the BmNPV-silkworm expression system is a cost-effective platform for producing TPO with high antigen activity.