Symbiotic efficiency and identification of rhizobia that nodulate cowpea in a Rhodic Eutrudox

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• 作者：Márcia Rufini (1)
  Michele Aparecida Pereira da Silva (1)
  Paulo Ademar Avelar Ferreira (1)
  Alice de Souza Cassetari (1)
  Bruno Lima Soares (1)
  Messias José Bastos de Andrade (2)
  Fatima Maria de Souza Moreira (1)
  
• 关键词：Biological nitrogen fixation ; Symbiosis ; Vigna unguiculata ; 16S rRNA ; Biodiversity
• 刊名：Biology and Fertility of Soils
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：50
• 期：1
• 页码：115-122
• 全文大小：169 KB
• 作者单位：Márcia Rufini (1)
  Michele Aparecida Pereira da Silva (1)
  Paulo Ademar Avelar Ferreira (1)
  Alice de Souza Cassetari (1)
  Bruno Lima Soares (1)
  Messias José Bastos de Andrade (2)
  Fatima Maria de Souza Moreira (1)
  
  1. Setor de Biologia, Microbiologia e Processos Biológicos do Solo, Departamento de Ciência do Solo, Universidade Federal de Lavras, Campus UFLA, 37200-000, Lavras, Minas Gerais, Brazil
  2. Setor de Grandes Culturas, Departamento de Agricultura, Universidade Federal de Lavras, Campus UFLA, 37200-000, Lavras, Minas Gerais, Brazil
  
• ISSN：1432-0789

文摘

Cowpea (Vigna unguiculata (L.) Walp.) is one of the most important crops in the north and northeast regions of Brazil, where it is grown under the subsistence regime, mainly because of its adaptation to the local edaphoclimatic conditions. This species is an important legume in Brazil because of its significant nutritional value in the diet of low-income populations. Moreover, it is able to benefit from the process of biological nitrogen fixation, which leads to a significant reduction in production costs. The objective of this research was to evaluate the agronomic efficiency of bacterial strains approved by the Ministry of Agriculture, Livestock and Supply for commercial inoculant production when in symbiosis with cowpea in a Rhodic Eutrudox in Lavras, MG, as well as to identify and verify the efficiency of the native populations that nodulate cowpea. Agronomic efficiency of nitrogen-fixing nodulating bacterial strains (UFLA 03-84, INPA 03-11B, and BR 3267) in symbiosis with cowpea was evaluated in the field. Subsequently, the efficiency and diversity of isolates from the nodules obtained in the field were evaluated under greenhouse conditions. The genetic diversity of the efficient isolates was determined by partial sequencing of the 16S rRNA gene. Grain yields obtained by the inoculation of seeds in the field with the strains were similar to those of the controls without inoculation with and without mineral N addition indicating the existence of efficient native populations. In the native community, we identified isolates with similar or higher efficiency than the strains approved for cowpea inoculation and the control that received mineral N. The isolates subjected to partial sequencing of the 16S rRNA gene were phylogenetically related to the Bradyrhizobium genus.