Close contact interferon-gamma response to the new PstS1_(285–374):CPF10: a preliminary 1-year follow-up study

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• 作者：Leonardo Silva de Araujo ; Nidai de Bárbara Moreira da Silva Lins…
• 关键词：M. tuberculosis ; Tuberculosis ; LTBI ; Interferon ; gamma ; Follow up
• 刊名：BMC Research Notes
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：10
• 期：1
• 全文大小：1679KB
• 刊物主题：Biomedicine, general; Medicine/Public Health, general; Life Sciences, general;
• 出版者：BioMed Central
• ISSN：1756-0500
• 卷排序：10

文摘

BackgroundThe available diagnostic tools for latent tuberculosis (TB) infection (LTBI) via interferon-gamma (IFN-g) release assays (IGRA) are based on ESAT6:CFP10 stimulation. However, the mycobacterial antigen PstS1 is also highly immunogenic and some of its fragments, such as PstS1_(285–374), have shown higher immunoreactivity in LTBI than in active TB. PstS1_(285–374), therefore, could increase the accuracy of the existing IGRA to detect LTBI. Thus, a new chimeric protein has recently been developed (PstS1_(285–374):CFP10) showing potential for LTBI screening of recent close contacts (rCt) exposed to Mycobacterium tuberculosis. The aim of this study was to analyze the PstS1_(285–374):CFP10 longitudinal IFN-g profile in comparison to ESAT6:CFP10 and full PstS1/CFP10 stimulation in a rCt cohort and correlate the responses to these in-house IGRA with any clinical changes/interventions that might occur.