Green Synthesis and Characterization of Biologically Active Silver Nanoparticles Using Perilla frutescens Leaf Extract
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- 作者：Muthuraman Pandurangan ; P. C. Nagajyothi ; Doo Hwan Kim…
- 关键词：Green synthesis ; Silver nanoparticles ; Human cervical cancer cell ; SRB assay ; Fluorescence
- 刊名：Journal of Cluster Science
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：28
- 期：1
- 页码：81-90
- 全文大小：
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Catalysis; Inorganic Chemistry; Physical Chemistry; Nanochemistry;
- 出版者：Springer US
- ISSN：1572-8862
- 卷排序：28
文摘
Silver nanoparticles (AgNPs) were synthesized using an aqueous Perilla leaf extract and evaluated for anticancer activity. These green -synthesized AgNPs were characterized using UV–Visible absorption spectroscopy, XRD, TEM, and SAED. The synthesized AgNPs were spherical and hexagonal in shape, and the average size of the NPs was ~23 nm. SRB assay investigated the anticancer activity of the AgNPs against human cervical cancer (HeLa) cells. The fluorescence and confocal microscopy studies were confirmed the DNA damage and apoptosis following nanoparticle treatment. Furthermore, the intracellular ROS was also increased after treatment. Taking all these data together, it is suggested that AgNPs may be a potential agent for the inhibition of cervical tumor cells.