The 23 kDa Polypeptide from Common Frog Lens: Isolation, Properties, and Cellular Localization

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• 作者：V. N. Simirskii and K. S. Aleinikova
• 关键词：tissue ; specific proteins ; crystallins ; chromatography ; lens ; common frog
• 刊名：Russian Journal of Developmental Biology
• 出版年：2002
• 出版时间：2002
• 年：2002
• 卷：33
• 期：4
• 页码：228-235
• 全文大小：343 KB

文摘

The major water-soluble polypeptide with molecular weight of approximately 23 kDa (the 23 kDa polypeptide) was identified in the lens of common frog Rana temporaria L. According to the gel filtration data, the peptide is a part of an oligomeric protein with molecular weight over than 300 kDa (-crystallin fraction). A highly pure fraction of the 23 kDa polypeptide was isolated by two-step ion-exchange chromatography and SDS electrophoresis and the specific antibodies were obtained. Immunohistochemistry showed the presence of the 23 kDa polypeptide in the cytoplasm of lens epithelial cells (including its central region) and in the zones neighboring the plasma membranes in the cortical fibers. The 23 kDa polypeptide was not found in the lens central zone (nucleus). It was also present in the retina (in the cells of inner nuclear layers), but not in the other tissues and organs of adult frog. Immunochemical analysis showed that the 23 kDa polypeptide was different from all known crystallins of frogs and other animals (bull, mouse, rat, and chicken). The nature of the 23 kDa polypeptide and the relation of its expression with the lens cell differentiation are discussed.