The WRKY Transcription Factor Family in Citrus: Valuable and Useful Candidate Genes for Citrus Breeding
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- 作者：M. Ayadi ; M. Hanana ; N. Kharrat ; H. Merchaoui…
- 关键词：Citrus ; WRKY ; Fruit acidification ; Essential oil biosynthesis ; Stress tolerance ; Zinc finger
- 刊名：Applied Biochemistry and Biotechnology
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：180
- 期：3
- 页码：516-543
- 全文大小：8,329 KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Biotechnology
Biochemistry - 出版者：Humana Press Inc.
- ISSN：1559-0291
- 卷排序：180
文摘
WRKY transcription factors belong to a large family of plant transcriptional regulators whose members have been reported to be involved in a wide range of biological roles including plant development, adaptation to environmental constraints and response to several diseases. However, little or poor information is available about WRKY’s in Citrus. The recent release of completely assembled genomes sequences of Citrus sinensis and Citrus clementina and the availability of ESTs sequences from other citrus species allowed us to perform a genome survey for Citrus WRKY proteins. In the present study, we identified 100 WRKY members from C. sinensis (51), C. clementina (48) and Citrus unshiu (1), and analyzed their chromosomal distribution, gene structure, gene duplication, syntenic relation and phylogenetic analysis. A phylogenetic tree of 100 Citrus WRKY sequences with their orthologs from Arabidopsis has distinguished seven groups. The CsWRKY genes were distributed across all ten sweet orange chromosomes. A comprehensive approach and an integrative analysis of Citrus WRKY gene expression revealed variable profiles of expression within tissues and stress conditions indicating functional diversification. Thus, candidate Citrus WRKY genes have been proposed as potentially involved in fruit acidification, essential oil biosynthesis and abiotic/biotic stress tolerance. Our results provided essential prerequisites for further WRKY genes cloning and functional analysis with an aim of citrus crop improvement.