Rhizobial diversity associated with the spontaneous legume Genista saharae in the northeastern Algerian Sahara

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• 作者：Khaled Chaïch ; Abdelkader Bekki ; Noureddine Bouras ; Michael D. Holtz…
• 关键词：Rhizobial diversity ; Spontaneous legume ; Genista saharae ; N2 ; fixation ; Housekeeping genes ; Algerian Sahara
• 刊名：Symbiosis
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：71
• 期：2
• 页码：111-120
• 全文大小：
• 刊物主题：Evolutionary Biology; Developmental Biology; Microbiology; Plant Sciences; Ecology;
• 出版者：Springer Netherlands
• ISSN：1878-7665
• 卷排序：71

文摘

Genista saharae is an indigenous shrub legume that spontaneously grows in the northeastern Algerian Sahara. It is known for efficient dune fixation and soil preservation against desertification, due to its drought tolerance and its contribution to sustainable nitrogen resources implemented by biological N₂-fixation. In this study, the root nodule bacteria of G. saharae were investigated using phenotypic and phylogenetic characterization. A total of 57 rhizobial strains were isolated from nodules from several sites in the hyper-arid region of Metlili and Taibet (east Septentrional Sahara). They all nodulate G. saharae species but they differed in their symbiotic efficiency and effectiveness. The genetic diversity was assessed by sequencing three housekeeping genes (atpD, recA and 16S rRNA). The majority of isolates (81 %) belonged to the genus Ensifer (previously Sinorhizobium), represented mainly by the species Ensifer meliloti. The next most abundant genera were Neorhizobium (17 %) with 3 different species: N. alkalisoli, N. galegae and N. huautlense and Mesorhizobium (1.75 %) represented by the species M. camelthorni. Most of the isolated strains tolerated up to 4 % (w/v) NaCl and grew at 45 °C. This study is the first report on the characterization of G. saharae microsymbionts in the Algerian Sahara.