Cloning and characterization of a protein elicitor Sm1 gene from Trichoderma harzianum

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• 作者：Rachel Silveira Freitas (1)
  Andrei Stecca Steindorff (1)
  Marcelo Henrique Soller Ramada (1)
  Saulo José Linhares de Siqueira (1)
  Eliane Ferreira Noronha (2)
  Cirano José Ulhoa (1)
  
• 关键词：Gene expression ; Protein elicitor ; Sm1 ; Trichoderma spp
• 刊名：Biotechnology Letters
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：36
• 期：4
• 页码：783-788
• 全文大小：540 KB
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• 作者单位：Rachel Silveira Freitas (1)
  Andrei Stecca Steindorff (1)
  Marcelo Henrique Soller Ramada (1)
  Saulo José Linhares de Siqueira (1)
  Eliane Ferreira Noronha (2)
  Cirano José Ulhoa (1)
  
  1. Department of Biochemistry and Molecular Biology, Federal University of Goiás (ICB II), Goiania, GO, 74690-900, Brazil
  2. Department of Cellular Biology, Brasília University (IB), Brasília, DF, 70910-900, Brazil
  
• ISSN：1573-6776

文摘

A small protein, cysteine-rich, designated SM1, produced by Trichoderma virens and Trichoderma atroviride, acts as elicitor for triggering plant defense reactions. We analyzed Sm1 gene expression of eight different strains of Trichoderma spp. grown on glucose, seeds or roots of beans. Regardless of the carbon source, T37 strain had significantly higher Sm1 expression and was chosen for further studies. When grown on different carbon sources, Sm1 expression was highest on galactose, bean seed, glucose and starch. Sm1 gene from T37 strain was cloned; it had a single exon, and encoded a protein of 138 amino acids, showing high sequence identity with some proteins belonging to the cerato-platanin family.