文摘
Two novel actinobacteria, designated strains NEAU-JM1T and NEAU-CL2T, were isolated from volcanic sediment and the rhizosphere soil of Paris polyphylla, respectively, collected from Jiling and Heilongjiang Province, China. A polyphasic study was carried out to establish the taxonomic positions of these strains. The 16S rRNA gene sequence analysis showed that the two novel isolates exhibit 99.5 % 16S rRNA gene sequence similarity with each other. The phylogenetic analysis based on 16S rRNA gene sequence of strain NEAU-JM1T showed it to be closely related to Catellatospora methionotrophica JCM 7543T (99.4 % sequence similarity), Catellatospora coxensis DSM 44901T (99.4 %), Catellatospora citrea DSM 44097T (99.3 %) and Catellatospora chokoriensis JCM 12950T (99.2 %), and that of strain NEAU-CL2T to C. citrea DSM 44097T (99.4 %), C. methionotrophica JCM 7543T (99.3 %), C. chokoriensis JCM 12950T (99.3 %) and C. coxensis DSM 44901T (99.2 %). However, the DNA–DNA hybridization value between strains NEAU-JM1T and NEAU-CL2T was 62.2 %, and the values between the two strains and their close phylogenetic relatives were also below 70 %. With reference to phenotypic characteristics, phylogenetic data and DNA–DNA hybridization results, the two strains can be distinguished from each other and their close phylogenetic relatives. Thus, strains NEAU-JM1T and NEAU-CL2T can be concluded to represent two novel species of the genus Catellatospora, for which the names Catellatospora vulcania sp. nov. and Catellatospora paridis sp. nov. are proposed. The type strains are NEAU-JM1T (=CGMCC 4.7174T = JCM 30054T) and NEAU-CL2T (=CGMCC 4.7236T = DSM 100519T), respectively.