文摘
Oleuropein, the major phenolic compound found in olive leaves and oil, exerts antioxidant, anti-inflammatory and anti-atherogenic effects and suppresses the adipocyte differentiation in vitro. Herein, we characterized molecular mechanisms underlying the anti-adipogenic effects of oleuropein on 3T3-L1 cells and adipocytes derived from stromal-vascular fraction of dorsolumbar and gonadal fat dissected from mice. We found that oleuropein (>100) decreased viability of preadipocytes proliferating in vitro and did not exerted any cytotoxic effects in post-confluent cells after induction of differentiation. Oleuropein (>100) inhibited adipocyte differentiation, suppressed gene expression of peroxisome proliferator-activated receptor (PPAR, CCAAT-/enhancer-binding protein sterol regulatory element-binding transcription factor 1c and fatty acid synthase. Furthermore, we tested ability of oleuropein to regulate of PPAR, PPAR or PPAR/PPARmediated lactamase expression in appropriate reporter gene assays. Oleuropein between 10 and 400 concentrations did not affect activity of PPARor PPAR Contrary, PPARactivity, either basal or rosiglitazone activated, was inhibited by oleuropein. Our data suggest that oleuropein exerts anti-adipogenic effect through direct inhibition of PPARtranscriptional activity.