Comparison of amylopectin structure and activities of key starch synthesis enzymes in the grains of rice single-segment substitution lines with different Wx alleles

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• 作者：Bin Teng ; Chen Zhang ; Ying Zhang ; Jingde Wu ; Zefu Li…
• 关键词：Rice ; Wx gene ; Allelic variation ; Amylopectin structure ; Starch synthesis enzyme ; Single ; segment substitution line
• 刊名：Plant Growth Regulation
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：77
• 期：2
• 页码：117-124
• 全文大小：464 KB
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• 作者单位：Bin Teng (1)
  Chen Zhang (2)
  Ying Zhang (1)
  Jingde Wu (1)
  Zefu Li (1)
  Zhixiang Luo (1)
  Jianbo Yang (1)
  
  1. Anhui Provincial Key Lab of Rice Genetics and Breeding, Institute of Rice Research, Anhui Academy of Agricultural Sciences, Hefei, 230031, People鈥檚 Republic of China
  2. College of Life Science, Anhui Agricultural University, Hefei, 230061, People鈥檚 Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5087

文摘

The Wx gene is the major gene controlling amylose synthesis in rice endosperm. So far, the Wx gene can be mainly classified into five common alleles, wx, Wx t, Wx g1, Wx g2, and Wx g3, according to their amylose phenotypes. Besides, the Wx allelic variation was also found to play an important role in regulating other physicochemical properties of rice starch. However, the specific physiological and starch structural mechanisms are not clear yet. With a set of single-segment substitution lines harboring five different Wx alleles, the genotype differences in amylopectin structure and dynamic changes of activities of key starch synthesis enzymes were investigated. The distinct Wx genotype difference in chain length distribution of amylopectin was confirmed by size exclusion chromatography. There were clear allelic differences in activities of starch synthesis enzymes during grain filling. The Wx allelic variation had the highest impact on the activities of starch branching enzyme, followed by pullulanase, and soluble starch synthase, while no influence on isoamylase activity. Differences in structural features of amylopectin and mean enzyme activities during grain filling period correlated well with most of the variations in starch property traits. These findings indicate that the Wx allelic variation may affect starch structure and physicochemical properties through selectively influencing activities of starch synthesis enzymes in developing rice grains. The present results will contribute to our understanding of the regulation of starch biosynthesis in rice endosperm. Keywords Rice Wx gene Allelic variation Amylopectin structure Starch synthesis enzyme Single-segment substitution line