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作者单位:Qingjun Guo (1) Xiangzhi Li (2) Congcong Shen (2) Songbai Zhang (3) Haizhi Qi (1) Ting Li (1) Minghui Yang (2)
1. Department of General Surgery, The Second Xiang-ya Hospital, Central South University, Changsha, 410011, China 2. College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China 3. Department of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde, 415000, China
刊物类别:Chemistry and Materials Science
刊物主题:Chemistry Analytical Chemistry Inorganic Chemistry Physical Chemistry Characterization and Evaluation Materials Monitoring, Environmental Analysis and Environmental Ecotoxicology
出版者:Springer Wien
ISSN:1436-5073
文摘
An electrochemical immunoassay is described for the detection of the protein biomarker mucin 1 (MUC-1) and of breast cancer cells of type MCF-7 where MUC-1 is overexpressed. The method is?based on the use of silver nanoclusters (Ag-NCs) acting as a signalling probe. The Ag-NCs were synthesized via chemical reduction in the presence of a?DNA strand with the sequence of 5-GCAGTTGATCCTTTGGATACCCTGG-C12-3- The strand contains mucin 1 aptamer (GCAGTTGATCCTTTGGATACCCTGG) that can specifically bind to MUC1 and the template (C12) for synthesis of Ag-NCs.?The assay involves the following steps: (1) Construction of an immunosensor by immobilizing the antibody against MUC-1 on a glassy carbon electrode; (2) addition of sample containing MUC-1; (3) addition of Ag-NCs; (4) signal amplification via silver enhancement process (deposition of metal silver on Ag-NCs); (5) measurement via square wave voltammetry. The current measured at a potential of 0.11?V (vs. SCE) is logarithmically related to the concentration of MUC-1 in the 1 to 500?nM range, with a detection limit of 0.5?nM. We also demonstrate that MCF-7 cancer cells can be detected by this method with high sensitivity (50 cells per mL) due to the presence of MUC-1 proteins on the cell surface.