Electrochemical immunoassay for the protein biomarker mucin 1 and for MCF-7 cancer cells based on signal enhancement by silver nanoclusters
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- 作者：Qingjun Guo ; Xiangzhi Li ; Congcong Shen ; Songbai Zhang ; Haizhi Qi…
- 关键词：Electrochemical ; Mucin 1 ; Breast cancer cell ; Silver nanoclusters ; Silver enhancement
- 刊名：Microchimica Acta
- 出版年：2015
- 出版时间：June 2015
- 年：2015
- 卷：182
- 期：7-8
- 页码：1483-1489
- 全文大小：491 KB
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27.Lin D - 作者单位：Qingjun Guo (1)
Xiangzhi Li (2)
Congcong Shen (2)
Songbai Zhang (3)
Haizhi Qi (1)
Ting Li (1)
Minghui Yang (2)
1. Department of General Surgery, The Second Xiang-ya Hospital, Central South University, Changsha, 410011, China
2. College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China
3. Department of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde, 415000, China - 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Analytical Chemistry
Inorganic Chemistry
Physical Chemistry
Characterization and Evaluation Materials
Monitoring, Environmental Analysis and Environmental Ecotoxicology - 出版者：Springer Wien
- ISSN：1436-5073
文摘
An electrochemical immunoassay is described for the detection of the protein biomarker mucin 1 (MUC-1) and of breast cancer cells of type MCF-7 where MUC-1 is overexpressed. The method is?based on the use of silver nanoclusters (Ag-NCs) acting as a signalling probe. The Ag-NCs were synthesized via chemical reduction in the presence of a?DNA strand with the sequence of 5-GCAGTTGATCCTTTGGATACCCTGG-C12-3- The strand contains mucin 1 aptamer (GCAGTTGATCCTTTGGATACCCTGG) that can specifically bind to MUC1 and the template (C12) for synthesis of Ag-NCs.?The assay involves the following steps: (1) Construction of an immunosensor by immobilizing the antibody against MUC-1 on a glassy carbon electrode; (2) addition of sample containing MUC-1; (3) addition of Ag-NCs; (4) signal amplification via silver enhancement process (deposition of metal silver on Ag-NCs); (5) measurement via square wave voltammetry. The current measured at a potential of 0.11?V (vs. SCE) is logarithmically related to the concentration of MUC-1 in the 1 to 500?nM range, with a detection limit of 0.5?nM. We also demonstrate that MCF-7 cancer cells can be detected by this method with high sensitivity (50 cells per mL) due to the presence of MUC-1 proteins on the cell surface.