文摘
A Gram-negative, strictly aerobic, non-motile, and rod-shaped bacterial strain designated UB-M7T was isolated from the intestine of a sea cucumber (Apostichopus japonicus) collected from Pohang in South Korea. Strain UB-M7T displayed optimal growth at 25?°C, pH 7.0-.5, and with 2.0-.0?% (w/v) NaCl. Phylogenetic analyses based on the 16S rRNA gene sequences showed that strain UB-M7T clustered with Phaeobacter arcticus DSM 23566T, Phaeobacter inhibens DSM 16374T, Phaeobacter gallaeciensis BS107T, and Phaeobacter leonis 306T, exhibiting 16S rRNA gene sequence similarity values of 96.8, 96.6, 96.4, and 96.2?%, respectively. Strain UB-M7T was found to exhibit the highest gyrB sequence similarity value of 80.6?% to the type strain of P. arcticus. The major respiratory quinone of strain UB-M7T was found to be ubiquinone 10 (Q-10). The major cellular fatty acids (>5?% of the total fatty acids) are summed features 8 (comprising C18:1 ω7c and/or C18:1 ω6c), 11-methyl C18:1 ω7c, and cyclo C19:0 ω8c. The DNA G+C content was found to be 58.5?mol% and DNA–DNA relatedness value with P. arcticus JCM 14644T was 17.2?±?2.4?%. The major polar lipids of strain UB-M7T were identified as phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine; one phospholipid, and three other lipids remain unidentified. Based on its phenotypic, phylogenetic, and chemotaxonomic properties it is concluded that strain UB-M7T represents a novel species in the genus Phaeobacter, for which the name Phaeobacter marinintestinus sp. nov. is proposed. The type strain is UB-M7T (=KCCM 43045T?=?JCM 19926T).