Pusillimonas caeni sp. nov., isolated from a sludge sample of a biofilm reactor
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- 作者：Long Jin ; So-Ra Ko ; Yingshun Cui ; Chang Soo Lee ; Hee-Mock Oh…
- 关键词：Biofilm reactor ; EBR ; 8 ; 1T ; Pusillimonas ; Pusillimonas caeni
- 刊名：Antonie van Leeuwenhoek
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：110
- 期：1
- 页码：125-132
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Microbiology; Medical Microbiology; Plant Sciences; Soil Science & Conservation;
- 出版者：Springer International Publishing
- ISSN：1572-9699
- 卷排序：110
文摘
A polyphasic taxonomic study was carried out on strain EBR-8-1T isolated from a biofilm reactor in Korea. The cells of the strain were Gram-stain negative, non-spore-forming, non-motile, and short rod-shaped. Comparative 16S rRNA gene sequence studies showed a clear affiliation of this strain with Betaproteobacteria, which showed high pairwise sequence similarities with Pusillimonas noertemannii BN9T (99.1 %), Pusillimonas soli MJ07T (97.3 %), Pusillimonas ginsengisoli DCY25T (97.2 %), and Pusillimonas harenae B201T (96.8 %). The phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a clear phylogenetic lineage within the genus Pusillimonas. The major fatty acids were identified as C16:0, C17:0 cyclo and C19:0 cyclo ω8c. The major cellular polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and an unidentified aminolipid. The respiratory quinone was identified as Q-8 and the genomic DNA G+C content was determined to be 63.3 mol%. On the basis of polyphasic evidence, it is proposed that strain EBR-8-1T should be placed in a new species, Pusillimonas caeni sp. nov. The type stain is EBR-8-1T (=KCTC 42353T = JCM 30463T).