Effect of house dust mite on neutrophil apoptosis by cytokine secretion in lymphocytes

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• 作者：Na Rae Lee ; Ji-Sook Lee ; Seung Yeop Baek ; Da Hye Kim…
• 关键词：Allergy ; Cytokine ; Lymphocytes ; Mite ; Neutrophils apoptosis
• 刊名：Molecular & Cellular Toxicology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：12
• 期：4
• 页码：371-379
• 全文大小：
• 刊物主题：Cell Biology; Pharmacology/Toxicology;
• 出版者：The Korean Society of Toxicogenomics and Toxicoproteomics
• ISSN：2092-8467
• 卷排序：12

文摘

Although extract of Dermatophagoides farinae (DF) alone had no effect on neutrophil apoptosis, it inhibited neutrophil apoptosis in neutrophils cocultured with lymphocytes in both normal and allergic subjects. DF showed a stronger inhibition on the apoptosis of allergic neutrophils cocultured with allergic lymphocytes than that of normal neutrophils cocultured with normal lymphocytes. DF induced the secretion of IL-6, IL-8, MCP-1, and GM-CSF of the normal and allergic lymphocytes. The cytokine secretion due to DF in allergic lymphocytes is higher than in normal lymphocytes. The cytokine secretion of normal and allergic lymphocytes induced by DF was suppressed by PAR2i, a PAR2 inhibitor, LY294002, a PI3K inhibitor, AKTi, an Akt inhibitor, PD98059, an ER K inhibitor, and BAY-11-7085, an NF-κB inhibitor. Phosphorylation of ERK induced by DF was suppressed by PAR2i, LY294002 and AKTi, and NF-κB activity due to DF was inhibited by PAR2i, LY294002, AKTi, and PD98059.