Effect of chitin hexamer and thuricin 17 on lignification-related and antioxidative enzymes in Soybean Plants

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• 作者：Woo -Jin Jung (1)
  Fazli Mabood (2)
  Alfred Souleimanov (2)
  Donald L Smith (2)
  
• 关键词：chitin hexamer ; peroxidase (POD) ; phenylalanine ammonia lyase (PAL) ; soybean ; Superoxide dismutase (SOD) ; thuricin 17 ; tyrosine ; ammonia lyase (TAL)
• 刊名：Journal of Plant Biology
• 出版年：2008
• 出版时间：March 2008
• 年：2008
• 卷：51
• 期：2
• 页码：145-149
• 全文大小：900KB
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• 作者单位：Woo -Jin Jung (1)
  Fazli Mabood (2)
  Alfred Souleimanov (2)
  Donald L Smith (2)
  
  1. Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University, 500-757, Gwangju, Korea
  2. Department of Plant Science, McGill University, Macdonald Campus, Lakeshore Road, St Anne-de-Bellevue, 21,111, Quebec, Canada, H9X 3V9
  

文摘

Inducers of disease resistance in crop plants have a role in sustainable agriculture. We describe a set of bacteriocins that can potentially improve plant growth by controlling specific pathogens and inducing generalized resistance. Solutions of the bacteriocin thuricin 17 and/or a chitin hexamer (a known inducer and positive control) were applied to leaves of two-week-old soybean plants, and levels of lignification-related and antioxidative enzymes were monitored. Phenyl ammonia lyase (PAL) activity in thuricin 17-treated leaves was highest at 60 h after treatment, being 61.8% greater than the control. PAL activity also was increased 18.1% at 72 h after treatment with the chitin hexamer. Tyrosine ammonia lyase (TAL) activity in leaves was 57.0% higher than the control at 48 h after treatment with thuricin 17, while such activity in chitin hexamer-treated leaves was increased by 23.8% at 72 h. At 36 h after treatment with the chitin hexamer or chitin hexamer + thuricin 17, the total concentration of phenolic compounds was 15.3 or 19.3%, respectively, greater than the control. At 72 h, total phenolic concentrations increased by 23.2 and 19%, respectively, in response to thuricin 17 and chitin hexamer + thuricin 17. POD activity in thuricin 17-treated leaves increased by 74.6 and 81.2% at 48 and 72 h, respectively, whereas SOD activity increased by 24.9 and 79.9%, respectively, in chitin hexamer- and thuricin 17-treated leaves at 48 h. A peroxidase isozyme (31 kDa isomer) was induced in thuricin 17-treated leaves at 60 h, while catalase (59 kDa isomer) was induced in chitin hexamer-treated leaves. PAGE showed that two major SOD bands (Fe-SODs) were produced by both types of treatment. Collectively, these results indicate that the bacteriocin thuricin 17 can act as an inducer of plant disease defenses (i.e., activated lignification-related enzymes, antioxidative enzymes, and related isozymes) and that this induction is similar, but not identical, to that of the chitin hexamer elicitor. Although treatment with thuricin 17 + chitin hexamer also induced those responses, it did not present a clear pattern of additivity or synergy.