Inducing goat pluripotent stem cells with four transcription factor mRNAs that activate endogenous promoters
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- 作者：Hao Chen ; Qisheng Zuo ; Yingjie Wang ; Jiuzhou Song ; Huilin Yang…
- 关键词：Goat embryonic fibroblasts ; Goat iPS cells ; Reprogramming ; mRNA
- 刊名：BMC Biotechnology
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：17
- 期：1
- 全文大小：5471KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Applied Microbiology; Biotechnology; Biochemical Engineering; Genetic Engineering; Plant Breeding/Biotechnology; Biomedical Engineering/Biotechnology;
- 出版者：BioMed Central
- ISSN：1472-6750
- 卷排序：17
文摘
BackgroundTraditional approaches for generating goat pluripotent stem cells (iPSCs) suffer from complexity and low preparation efficiency. Therefore, we tried to derive goat iPSCs with a new method by transfecting exogenous Oct4, Sox2, Klf4 and c-Myc mRNAs into goat embryonic fibroblasts (GEFs), and explore the mechanisms regarding the transcription regulation of the reprogramming factors in goat iPSCs induction.