Diagnosis of a new variant of soybean yellow mottle mosaic virus with extended host-range in India

详细信息    查看全文

• 作者：Nagamani Sandra ; Alok Kumar ; Prachi Sharma ; Reetika Kapoor…
• 关键词：Carmovirus ; Blackgram mottle virus ; Soybean yellow mottle mosaic virus ; Host reaction ; ELISA ; Recombinant coat protein ; Western blot
• 刊名：Indian Journal of Virology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：26
• 期：4
• 页码：304-314
• 全文大小：1,877 KB
• 参考文献：1.Clark MF, Bar-Joseph M. Enzyme immunosorbent assays in plant virology. In: Maramorosch K, Koprowski H, editors. Methods in virology. New York: Academic Press; 1984. p. 51-5.
  2.Gasteiger E, Hoogland C, Gattiker A, Duvaud S, Wilkins MR, Appel RD, Bairoch A. Protein identification and analysis tools on the EXPASY server. In: Walker JM, editor. The proteomics protocols handbook. Totowa: Humana press; 2005. p. 571-07.CrossRef
  3.Hall TA. BioEdit a user-friendly biological sequence alignment editor and analysis programme for windows 95/98/NT. Nucleic Acids Symp Ser. 1999;41:95-.
  4.Jain RK, Pandey AN, Krishna Reddy M, Mandal B. Immunodiagnosis of groundnut and watermelon bud necrosis viruses using polyclonal antiserum to recombinant nucleocapsid protein of Groundnut bud necrosis virus. J Virol Methods. 2005;130:162-.CrossRef PubMed
  5.Kaiser WJ, Mossahebi H. Natural infection of mungbean by Bean common mosaic virus. Phytopathology. 1974;64:1209-4.CrossRef
  6.King AMQ, Adams MJ, Carstens E, Lefkowitz EJ. Virus taxonomy: classification and nomenclature of viruses: ninth report of the international committee on taxonomy of viruses. Amsterdam: Elsevier Academic press; 2012.
  7.Li S, Moon JS, Lee SH, Domier LL. First report of Soybean yellow mottle mosaic virus in soybean in North America. Plant Dis. 2009;93:1214.CrossRef
  8.Mandal B, Varma A. Differentiation of natural variants of mungbean yellow mosaic geminivirus by host reactions and DNA–DNA hybridization. Int J Trop Plant Dis. 1996;14(2):189-02.
  9.Mandal B, Kumar A, Rani P, Jain RK. Complete genome sequence, phylogenetic relationships and molecular diagnosis of an Indian isolate of Potato Virus X. J Phytopathol. 2012;160:1-.CrossRef
  10.Mink GI, Keswani CL. First report of Cowpea mild mottle virus on bean and mungbean in Tanzania. Plant Dis. 1987;71:557.CrossRef
  11.Nam M, Kim SM, Domier LL, Koh S, Moon JK, Choi HS, Kim HG, Moon JS, Lee SH. Nucleotide sequence and genome organization of a newly identified member of the genus Carmovirus, Soybean yellow mottle mosaic virus from soybean. Arch Virol. 2009;154:1679-4.CrossRef PubMed
  12.Phatak HC. Seed borne plant viruses-identification and diagnosis in seed health testing. Seed Sci Technol. 1974;2:3-55.
  13.Raikhy G, Hallan V, Kulshrestha S, Zaidi AA. Polyclonal antibodies to the coat protein of Carnation etched ring virus expressed in bacterial system: production and use in immunodiagnosis. J Phytopathol. 2007;155:616-2.CrossRef
  14.Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Laboratory; 1989.
  15.Scott HA, Phatak HC. Properties of blackgram mottle virus. Phytopathology. 1979;69:346-.CrossRef
  16.Soumya K, Yogita M, Prasanthi Y, Anitha K, Kishor PBK, Jain RK, Mandal B. Molecular characterization of Indian isolate of Peanut mottle virus and immunodiagnosis using bacterial expressed core capsid protein. Virus Dis. 2014;25:331-.CrossRef
  17.Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Bio Evol. 2013;30:2725-.CrossRef
  18.Thien XHO, Bhat AI, Jain RK. Mungbean necrosis disease caused by a strain of Groundnut bud necrosis tospovirus. Indian Phytopathol. 2003;56:54-0.
  19.Varma A, Krishnareddy M, Malathi VG. Influence of the amount of blackgram mottle virus in different tissues on transmission through the seeds of Vigna mungo. Plant Pathol. 1992;41(3):274-1.CrossRef
  
• 作者单位：Nagamani Sandra (1)
  Alok Kumar (1)
  Prachi Sharma (2)
  Reetika Kapoor (1)
  Rakesh Kumar Jain (1)
  Bikash Mandal (1)
  
  1. Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute (IARI), New Delhi, 110012, India
  2. Division of Plant Pathology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu (SKUAST-J), Jammu and Kashmir, 180009, India
  
• 刊物主题：Microbiology; Life Sciences, general; Biochemistry, general; Protein Structure; Cell Biology;
• 出版者：Springer India
• ISSN：0974-0120

文摘

Soybean yellow mottle mosaic virus (SYMMV, genus Carmovirus) was previously known to occur in South Korea and USA causing bright yellow mosaic in soybean. In this study, SYMMV (Car-Mb14 isolate) was isolated from mungbean (Vigna radiata) exhibiting mild mottling and puckering symptoms in the experimental field at Indian Agricultural Research Institute, New Delhi during 2012. The virus isolate, Car-Mb14 induced veinal mottling, mild mottling, chlorotic blotching, local and systemic necrosis in soybean, mungbean, blackgram, French bean and guar bean, respectively. The symptomatology of the present isolate of SYMMV was different from the previously reported South Korean isolate, as the later did not induce symptoms in any of the above legumes other than soybean. The present isolate was phylogenetically distinct and shared 90-3 % sequence identity in coat protein (CP) of 52 SYMMV isolates reported from Korea and USA. In order to know the serological relationships, the CP gene of the present isolate was over expressed as a 39 kDa protein in E. coli and an antiserum of 1:16,000 titer against the recombinant CP was produced. Serological cross reactivity analysis revealed that SYMMV was serologically related to blackgram mottle virus but not to cowpea mottle virus, the other legume infecting carmoviruses. The antiserum was used to detect prevalence of SYMMV in legume crops by ELISA. Out of 145 field samples of legumes (mungbean, blackgram, French bean and soybean) collected from different places in India, SYMMV was detected only in 16 samples of mungbean and one sample of blackgram. The natural infection of SYMMV in mungbean and blackgram was further confirmed based on CP gene sequence. This study provides evidence of occurrence of a new variant of SYMMV with distinct symptom phenotype and extended host-range in India. Keywords Carmovirus Blackgram mottle virus Soybean yellow mottle mosaic virus Host reaction ELISA Recombinant coat protein Western blot