Depression of HspA2 in human testis is associated with spermatogenic impairment and fertilization rate in ICSI treatment for azoospermic individuals

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• 作者：Yonghong Tian (1)
  Fengbin Zhang (1)
  Xinzong Zhang (2)
  Lejun Li (1)
  Liquang Wang (1)
  Biwei Shi (1)
  Jian Xu (1)
  
• 关键词：Spermatogenesis ; Heat shock protein A2 ; Immunohistochemistry ; Fertilization rate ; ICSI
• 刊名：Journal of Assisted Reproduction and Genetics
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：31
• 期：12
• 页码：1687-1693
• 全文大小：671 KB
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  13. Dix DJ, Allen JW, Collins BW, Mori C, Nakamura N, Poorman-Allen P, et al. Targeted gene disruption of Hsp70-2 results in failed meiosis, germ cell apoptosis, and male infertility. Proc Natl Acad Sci U S A. 1996;93(8):3264-. CrossRef
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  15. Cedenho AP, Lima SB, Cenedeze MA, Spaine DM, Ortiz V, Oehninger S. Oligozoospermia and heat-shock protein expression in ejaculated spermatozoa. Hum Reprod. 2006;21(7):1791-. CrossRef
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• 作者单位：Yonghong Tian (1)
  Fengbin Zhang (1)
  Xinzong Zhang (2)
  Lejun Li (1)
  Liquang Wang (1)
  Biwei Shi (1)
  Jian Xu (1)
  
  1. Reproductive Medicine Center, Department of Reproductive Endocrinology, Women’s Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310006, China
  2. Zhejiang Institute of Planned Parenthood Research and Zhejiang Human Sperm Bank, Hangzhou, 310012, China
  
• ISSN：1573-7330

文摘

Purpose Heat shock protein A2 (HspA2) expression was quantitatively measured in human testis and its relationship with the spermatogenetic status and laboratory outcomes of intracytoplasmic sperm injection (ICSI) was investigated. Methods Testicular tissues of azoospermia men were divided into four groups according to histopahtology: normal spermatogenesiss, hypospermatogenesis, maturation arrest and Sertoli cell-only syndrome (SCOS). HspA2 immunostaining was measured by Image Pro-Plus (IPP) and laboratory outcomes were calculated. The regression analysis between HspA2 expression and Johnsen score of as well as fertilization, cleavage and high quality embryo rate was performed. Results HspA2 was strongly present in the cytoplasm of spermatocytes and spermatides in normal testis. However, hypospermatogenesis and maturation arrest testicular tissues demonstrated light staining and no staining for SCOS. Quantitative image analysis showed that there were significant differences among groups (P--.000 & P--.001). HspA2 exspression was founded significantly correlated spermatogenetic status (R2--.726, P--.000) as well as fertilization rate in ICSI (R2--.569, P--.000). Conclusions The fertilization rate with ICSI is associated with HspA2 expression in the testis from which sperm retrieved and the alteration of HspA2 expression has been involved in spermatogenic impairment.