Identification of Plasmalogen Cardiolipins from Pectinatus by Liquid Chromatographyigh Resolution Electrospray Ionization Tandem Mass Spectrometry

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• 作者：Tom ezanka (1)
  Dagmar Matoulkov(2)
  Lucie Kyselov(3)
  Karel Sigler (1)
  
• 关键词：Pectinatus ; Plasmalogens ; Cardiolipins ; Liquid chromatographylectrospray tandem mass spectrometry
• 刊名：Lipids
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：48
• 期：12
• 页码：1237-1251
• 全文大小：
• 作者单位：Tom ezanka (1)
  Dagmar Matoulkov(2)
  Lucie Kyselov(3)
  Karel Sigler (1)
  
  1. Institute of Microbiology, Academy of Sciences of the Czech Republic, Vek1083, 14220, Prague, Czech Republic
  2. Research Institute of Brewing and Malting, Lov511/15, 12000, Prague, Czech Republic
  3. Department of Biotechnology, Institute of Chemical Technology Prague, Technick5, 16628, Prague, Czech Republic
  
• ISSN：1558-9307

文摘

High resolution electrospray ionization tandem mass spectrometry (HR-ESIS/MS) was used to analyze cardiolipins (Ptd2Gro) including their plasmalogen forms from three species of the anaerobic beer-spoilage bacterial genus Pectinatus. Cardiolipins including their plasmalogens were analyzed by HR-ESIS/MS on Orbitrap and almost 100 cardiolipins (i.e. tetra-acyltd2Gro, plasmenyl-tri-acyllsPtd2Gro, and di-plasmenyl-di-acylls2Ptd2Gro) of three classes were identified. The structures of the molecular species that consist of various regioisomers and structurally similar compounds were revealed in detail. The high resolution mass spectrometry allowed the unambiguous structural assignment of Ptd2Gro, PlsPtd2Gro, and Pls2Ptd2Gro in the three species of Pectinatus, which contain predominantly odd numbered fatty acids.