Identification of beer spoilage microorganisms using the MALDI Biotyper platform
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- 作者：Michelle Elizabeth Turvey ; Florian Weiland…
- 关键词：Beer spoilage microorganisms ; Biotyper ; Quality control ; Mass spectrometry ; MALDI
- 刊名：Applied Microbiology and Biotechnology
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：100
- 期：6
- 页码：2761-2773
- 全文大小：1,654 KB
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Florian Weiland (1) (2)
Jon Meneses (3)
Nick Sterenberg (3)
Peter Hoffmann (1) (2)
1. Adelaide Proteomics Centre, The University of Adelaide, Adelaide, Australia
2. Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, Australia
3. Coopers Brewery Ltd, Adelaide, Australia - 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Biotechnology
Microbiology
Microbial Genetics and Genomics - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-0614
文摘
Beer spoilage microorganisms present a major risk for the brewing industry and can lead to cost-intensive recall of contaminated products and damage to brand reputation. The applicability of molecular profiling using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) in combination with Biotyper software was investigated for the identification of beer spoilage microorganisms from routine brewery quality control samples. Reference mass spectrum profiles for three of the most common bacterial beer spoilage microorganisms (Lactobacillus lindneri, Lactobacillus brevis and Pediococcus damnosus), four commercially available brewing yeast strains (top- and bottom-fermenting) and Dekkera/Brettanomyces bruxellensis wild yeast were established, incorporated into the Biotyper reference library and validated by successful identification after inoculation into beer. Each bacterial species could be accurately identified and distinguished from one another and from over 5600 other microorganisms present in the Biotyper database. In addition, wild yeast contaminations were rapidly detected and distinguished from top- and bottom-fermenting brewing strains. The applicability and integration of mass spectrometry profiling using the Biotyper platform into existing brewery quality assurance practices within industry were assessed by analysing routine microbiology control samples from a local brewery, where contaminating microorganisms could be reliably identified. Brewery-isolated microorganisms not present in the Biotyper database were further analysed for identification using LC-MS/MS methods. This renders the Biotyper platform a promising candidate for biological quality control testing within the brewing industry as a more rapid, high-throughput and cost-effective technology that can be tailored for the detection of brewery-specific spoilage organisms from the local environment.