The assessment of host and bacterial proteins in sputum from active pulmonary tuberculosis

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• 作者：Hsin-Chih Lai ; Yu-Tze Horng ; Pen-Fang Yeh ; Jann-Yuan Wang…
• 关键词：Mycobacterium tuberculosis ; tuberculosis ; acid ; fast bacilli ; sputum ; transferrin ; urease
• 刊名：Journal of Microbiology
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：54
• 期：11
• 页码：761-767
• 全文大小：281 KB
• 刊物主题：Microbiology;
• 出版者：Springer Netherlands
• ISSN：1976-3794
• 卷排序：54

文摘

Pulmonary tuberculosis (TB) is caused by Mycobacterium tuberculosis. The protein composition of sputum may reflect the immune status of the lung. This study aimed to evaluate the protein profiles in spontaneous sputum samples from patients with active pulmonary TB. Sputum samples were collected from patients with pulmonary TB and healthy controls. Western blotting was used to analyze the amount of interleukin 10 (IL-10), interferon-gamma (IFN-γ), IL-25, IL-17, perforin-1, urease, albumin, transferrin, lactoferrin, adenosine deaminase (also known as adenosine aminohydrolase, or ADA), ADA-2, granzyme B, granulysin, and caspase-1 in sputum. Results of detection of IL-10, IFN-γ, perforin-1, urease, ADA2, and caspase-1, showed relatively high specificity in distinguishing patients with TB from healthy controls, although sensitivities varied from 13.3% to 66.1%. By defining a positive result as the detection of any two proteins in sputum samples, combined use of transferrin and urease as markers increased sensitivity to 73.2% and specificity to 71.1%. Furthermore, we observed that the concentration of transferrin was proportional to the number of acid-fast bacilli detected in sputum specimens. Detection of sputum transferrin and urease was highly associated with pulmonary TB infection. In addition, a high concentration of transferrin detected in sputum might correlate with active TB infection. This data on sputum proteins in patients with TB may aid in the development of biomarkers to assess the severity of pulmonary TB.