Further improvement in ganoderic acid production in static liquid culture of Ganoderma lucidum by integrating nitrogen limitation and calcium ion addition

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• 作者：Huan-Jun Li ; De-Huai Zhang ; Li-Liang Han ; Xuya Yu…
• 关键词：Ganoderic acid production ; Ganoderma lucidum ; Bioprocessing strategy ; Medicinal mushroom fermentation ; Bioactive secondary metabolites
• 刊名：Bioprocess and Biosystems Engineering
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：39
• 期：1
• 页码：75-80
• 全文大小：520 KB
• 参考文献：1.Paterson RRM (2006) Ganoderma—a therapeutic fungal biofactory. Phytochemistry 67:1985–2001CrossRef
  2.Xu JW, Ji SL, Li HJ, Ren MF, Duan YQ, Dang LZ, Mo MH (2015) Increased polysaccharide production and biosynthetic gene expressions in a submerged culture of Ganoderma lucidum by the overexpression of the homologous alpha-phosphoglucomutase gene. Bioproc Biosys Eng 38:399–405CrossRef
  3.Boh B (2013) Ganoderma lucidum: a potential for biotechnological production of anti-cancer and immunomodulatory drugs. Recent Pat Anticancer Drug Discov 8:255–287CrossRef
  4.Xu JW, Zhao W, Zhong JJ (2010) Biotechnological production and application of ganoderic acids. Appl Microbiol Biotechnol 87:457–466CrossRef
  5.Upadhyay M, Shrivastava B, Jain A, Kidwai M, Kumar S, Gomes J, Goswami DG, Panda AK, Kuhad RC (2014) Production of ganoderic acid by Ganoderma lucidum RCKB-2010 and its therapeutic potential. Ann Microbiol 64:839–846CrossRef
  6.Chen NH, Liu JW, Zhong JJ (2010) Ganoderic acid T inhibits tumor invasion in vitro and in vivo through inhibition of MMP expression. Pharmacol Rep 62:150–163CrossRef
  7.Tang W, Liu JW, Zhao WM, Wei DZ, Zhong JJ (2006) Ganoderic acid T from Ganoderma lucidum mycelia induces mitochondria mediated apoptosis in lung cancer cells. Life Sci 80:205–211CrossRef
  8.Xu K, Liang X, Gao F, Zhong JJ, Liu JW (2012) Antimetastatic effect of ganoderic acid T in vitro through inhibition of cancer cell invasion. Process Biochem 45:1261–1267CrossRef
  9.Shi L, Ren A, Mu D, Zhao MW (2010) Current progress in the study on biosynthesis and regulation of ganoderic acids. Appl Microbiol Biotechnol 88:1243–1251CrossRef
  10.Wang JL, Gu TY, Zhong JJ (2012) Enhanced recovery of antitumor ganoderic acid T from Ganoderma lucidum mycelia by novel chemical conversion strategy. Biotech Bioeng 109:754–762CrossRef
  11.Diamantopoulou P, Papanikolaou S, Komaitis M, Aggelis G, Philippoussis A (2014) Patterns of major metabolites biosynthesis by different mushroom fungi grown on glucose-based submerged cultures. Bioproc Biosys Eng 37:1380–1455
  12.Wagner R, Mitchell DA, Sassaki GL, De Almeida Amazonas MAL, Berovic M (2003) Current techniques for the cultivation of Ganoderma lucidum for the production of biomass, ganoderic acid and polysaccharides. Food Technol Biotechnol 41:371–382
  13.Wei ZH, Duan YY, Qian YQ, Guo XF, Li YJ, Jin SH, Zhou ZX, Shan SY, Wang CR, Chen XJ (2014) Screening of Ganoderma strains with high polysaccharides and ganoderic acid contents and optimization of the fermentation medium by statistical methods. Bioproc Biosys Eng 37:1789–1797CrossRef
  14.You BJ, Lee HZ, Chung KR, Lee MH, Huang MJ, Tien N, Chan CW, Kuo YH (2012) Enhanced production of ganoderic acids and cytotoxicity of Ganoderma lucidum using solid-medium culture. Biosci Biotech Bioch 76:1529–1534CrossRef
  15.Liang CX, Li YB, Xu JW, Wang JL, Miao XY, Tang YJ, Gu T, Zhong JJ (2010) Enhanced biosynthetic gene expressions and production of ganoderic acids in static liquid culture of Ganoderma lucidum under phenobarbital induction. Appl Microbiol Biotechnol 86:1367–1374CrossRef
  16.Ren A, Li XB, Miao ZG, Shi L, Jiang AL, Zhao MW (2014) Transcript and metabolite alterations increase ganoderic acid content in Ganoderma lucidum using acetic acid as an inducer. Biotechnol Let 36:2529–2536CrossRef
  17.Ren A, Qin L, Shi L, Dong X, Mu DS, Li YX, Zhao MW (2010) Methyl jasmonate induces ganoderic acid biosynthesis in the basidiomycetous fungus Ganoderma lucidum. Bioresour Technol 101:6785–6790CrossRef
  18.Xu YN, Xia XX, Zhong JJ (2013) Induced effect of Na plus on ganoderic acid biosynthesis in static liquid culture of Ganoderma lucidum via calcineurin signal transduction. Biotechnol Bioeng 110:1913–1923CrossRef
  19.You BJ, Lee MH, Tien N, Lee MS, Hsieh HC, Tseng LH, Chung YL, Lee HZ (2013) A novel approach to enhancing ganoderic acid production by Ganoderma lucidum using apoptosis induction. PLoS One 8:e53616CrossRef
  20.Zhang JM, Zhong JJ, Geng AL (2014) Improvement of ganoderic acid production by fermentation of Ganoderma lucidum with cellulase as an elicitor. Process Biochem 49:1580–1586CrossRef
  21.Tang YJ, Zhang W, Zhong JJ (2009) Performance analyses of a pH-shift and DOT-shift integrated fed-batch fermentation process for the production of ganoderic acid and Ganoderma polysaccharides by medicinal mushroom Ganoderma lucidum. Bioresour Technol 100:1852–1859CrossRef
  22.Zhu LW, Zhong JJ, Tang YJ (2010) Multi-fed batch culture integrated with three-stage light irradiation and multiple additions of copper ions for the hyperproduction of ganoderic acid and Ganoderma polysaccharides by the medicinal mushroom Ganoderma lucidum. Process Biochem 15:1904–1911CrossRef
  23.Xu JW, Xu YN, Zhong JJ (2012) Enhancement of ganoderic acid accumulation by overexpression of an N-terminally truncated 3-hydroxy-3-methylglutaryl coenzyme A reductase gene in the basidiomycete Ganoderma lucidum. Appl Environ Microbiol 78:7968–7976CrossRef
  24.Yu X, Ji SL, He YL, Ren MF, Xu JW (2014) Development of an expression plasmid and its use in genetic manipulation of Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum (higher basidiomycetes). Int J Med Mushrooms 16:161–168CrossRef
  25.Zhou JS, Ji SL, Ren MF, He YL, Jing XR, Xu JW (2014) Enhanced accumulation of individual ganoderic acids in a submerged culture of Ganoderma lucidum by the overexpression of squalene synthase gene. Biochem Eng J 90:178–183CrossRef
  26.Xu JW, Xu YN, Zhong JJ (2010) Production of individual ganoderic acids and expression of biosynthetic genes in liquid static and shaking cultures of Ganoderma lucidum. Appl Microbiol Biotechnol 85:941–948CrossRef
  27.Zhao W, Xu JW, Zhong JJ (2011) Enhanced production of ganoderic acids in static liquid culture of Ganoderma lucidum under nitrogen-limiting conditions. Bioresour Technol 102:8185–8190CrossRef
  28.Xu YN, Zhong JJ (2012) Impacts of calcium signal transduction on the fermentation production of antitumor ganoderic acids by medicinal mushroom Ganoderma lucidum. Biotech Adv 30:1301–1308CrossRef
  29.Sun L, Ren L, Zhuang X, Ji X, Yan J, Huang H (2014) Differential effects of nutrient limitations on biochemical constituents and docosahexaenoic acid production of Schizochytrium sp. Bioresour Technol 159:199–206CrossRef
  30.Wilkin DJ, Edwards PA (1992) Calcium ionophore treatment impairs the sterol-mediated suppression of 3-hydroxy-3-methylglutaryl-coenzyme A reductase, 3-hydroxy-3-methylglutaryl-coenzyme A synthase and farnesyl diphosphate synthetase. J Biol Chem 267:2831–2836
  31.Maury J, Asadollahi MA, Møller K, Clark A, Nielsen J (2005) Microbial isoprenoid production: an example of green chemistry through metabolic engineering. Adv Biochem Eng Biotechnol 100:19–51
  32.Ohyama K, Suzuki M, Masuda K, Yoshida S, Muranaka T (2007) Chemical phenotypes of the hmg1 and hmg2 mutants of Arabidopsis demonstrate the in-planta role of HMG-CoA reductase in triterpene biosynthesis. Chem Pharm Bull 55:1518–1521CrossRef
  33.Seo JW, Jeong JH, Shin CG, Lo SC, Han SS, Yu KW, Harada E, Han JY, Choi YE (2005) Overexpression of squalene synthase in Eleutherococcus senticosus increases phytosterol and triterpene accumulation. Phytochemistry 66:869–877CrossRef
  
• 作者单位：Huan-Jun Li (1)
  De-Huai Zhang (1)
  Li-Liang Han (1)
  Xuya Yu (1)
  Peng Zhao (1)
  Tao Li (1)
  Jian-Jiang Zhong (2)
  Jun-Wei Xu (1)
  
  1. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China
  2. State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dong-chuan Road, Shanghai, 200240, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Industrial Chemistry and Chemical Engineering
  Industrial and Production Engineering
  Waste Management and Waste Technology
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  Food Science
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1615-7605

文摘

To further improve the ganoderic acid (GA) production, a novel integrated strategy by combining nitrogen limitation and calcium ion addition was developed. The effects of the integrated combination on the content of GA-T (one powerful anticancer compound), their intermediates (squalene and lanosterol) and on the transcription levels of GA biosynthetic genes in G. lucidum fermentation were investigated. The maximum GA-T content with the integrated strategy were 1.87 mg/ 100 mg dry cell weight, which was 2.1–4.2 fold higher than that obtained with either calcium ion addition or nitrogen limitation alone, and it is also the highest record as ever reported in submerged fermentation of G. lucidum. The squalene content was increased by 3.9- and 2.2-fold in this case compared with either individual strategy alone. Moreover, the transcription levels of the GA biosynthetic genes encoding 3-hydroxy-3-methyglutaryl coenzyme A reductase and lanosterol synthase were also up-regulated by 3.3–7.5 and 1.3–2.3 fold, respectively.