Development and characterization of thirty-three microsatellite markers for the Patagonian sprat, Sprattus fuegensis (Jenyns, 1842), using paired-end Illumina shotgun sequencing

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• 作者：Sandra Ferrada-Fuentes (1)
  Ricardo Galleguillos (1)
  Cristian B. Canales-Aguirre (1)
  Cara N. Love (2)
  Kenneth L. Jones (3)
  Stacey L. Lance (2)
  
• 关键词：Sprattus ; Illumina ; Microsatellite ; PAL_FINDER ; PCR primers ; SSR
• 刊名：Conservation Genetics Resources
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：6
• 期：4
• 页码：833-836
• 全文大小：160 KB
• 参考文献：1. Aranis A, Meléndez R, Germán P, Cerna TF (2007) / Sprattus fuegensis en aguas interiores de Chiloé, Chile (Osteichthyes: Clupeiformes: Clupeidae). Gayana 71:102-13
  2. Canales-Aguirre CB, Ferrada S, Hernández CE, Galleguillos R (2010) Population structure and demographic history of / Genypterus blacodes using microsatellite loci. Fish Res 106:102-06. doi:10.1016/j.fishres.2010.06.010 CrossRef
  3. Castoe TA, Poole AW, de Koning APJ et al (2012) Rapid microsatellite identification from Illumina paired-end genomic sequencing in two birds and a snake. PLoS One 7:e30953. doi:10.1371/journal.pone.0030953 CrossRef
  4. Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288-95. doi:10.1111/j.1471-8286.2005.01155.x CrossRef
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• 作者单位：Sandra Ferrada-Fuentes (1)
  Ricardo Galleguillos (1)
  Cristian B. Canales-Aguirre (1)
  Cara N. Love (2)
  Kenneth L. Jones (3)
  Stacey L. Lance (2)
  
  1. Laboratorio de Genética y Acuicultura, Departamento de Oceanografía, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Casilla 160-C, Concepción, Chile
  2. Savannah River Ecology Laboratory, University of Georgia, Aiken, SC, 29802, USA
  3. Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO, 80045, USA
  
• ISSN：1877-7260

文摘

We isolated and characterized a total of 33 microsatellite loci from the Patagonian sprat Sprattus fuegensis, a recent exploited marine resource with a conservation status unknowing. Loci were screened in 24 individuals from the inshore waters of the Aysén Fjord, Chile. The number of alleles per locus ranged from 7 to 24, observed heterozygosity ranged from 0.217 to 0.875, and the probability of identity values ranged from 0.006 to 0.133. These new loci will provide tools for examining population genetic structure, estimating effective population size and provide information to fisheries management and conservation.