Kaur S, Harjai K, Chhibber S. 2012. Methicillin-resistant Staphylococcus aureus phage plaque size enhancement using sublethal concentrations of antibiotics. Appl Environ Microbiol, 78: 8227–8233.PubMed PubMedCentral CrossRef
Łobocka M, Hejnowicz MS, Dąbrowski K, Gozdek A, Kosakowski J, Witkowska M, Ulatowska MI, Weber-Dąbrowska B, Kwiatek M, Parasion S. 2012. Genomics of staphylococcal Twort-like phages—potential therapeutics of the post-antibiotic era. Adv Virus Res, 83: 143–216.PubMed CrossRef
Méric G, Miragaia M, de Been M, Yahara K, Pascoe B, Mageiros L, Mikhail J, Harris LG, Wilkinson TS, Rolo J, et al. 2015. Ecological Overlap and Horizontal Gene Transfer in Staphylococcus aureus and Staphylococcus epidermidis. Genome Biol Evol, 7: 1313–1328.PubMed PubMedCentral CrossRef
Moellering RC. 2012. MRSA: the first half century. J Antimicrob Chemother, 67: 4–11.PubMed CrossRef
Overbeek R, Olson R, Pusch GD, Olsen GJ, Davis JJ, Disz T, Edwards RA, Gerdes S, Parrello B, Shukla M, et al. 2014. The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST). Nucleic Acids Res, 42: D206–D214.PubMed PubMedCentral CrossRef
Rosenthal VD, Maki DG, Mehta Y, Leblebicioglu H, Memish ZA, Al-Mousa HH, Balkhy H, Hu B, Alvarez-Moreno C, Medeiros EA, et al. 2014. International Nosocomial Infection Control Consortium (INICC) report, data summary of 43 countries for 2007-2012. Device-associated module. Am J Infect Control, 42: 942–956.PubMed CrossRef
Stewart CR, Casjens SR, Cresawn SG, Houtz JM, Smith AL, Ford ME, Peebles CL, Hatfull GF, Hendrix RW, Huang WM. 2009. The genome of bacillus subtilis bacteriophage SPO1. J Mol Biol, 388: 48–70.PubMed PubMedCentral CrossRef
Zaballos A, Mellado RP, Salas M. 1988. Initiation of phage φ 29 DNA replication by mutants with deletions at the amino end of the terminal protein. Gene, 63: 113–121.PubMed CrossRef
Zhang X, Wang Y, Li S, an X, Pei G, Yong H, Hang F, Mi Z, Zhang Z, Wei W. 2015. A novel termini analysis theory using HTS data alone for the identification of Enterococcus phage EF4-like genome termini. Bmc Genomics, 16: 1–11.CrossRef

作者单位：Xianglilan Zhang (1)
Huaixing Kang (1) (2)
Yuyuan Li (1)
Xiaodong Liu (1)
Yu Yang (1)
Shasha Li (1)
Guangqian Pei (1)
Qiang Sun (1)
Peng Shu (1)
Zhiqiang Mi (1)
Yong Huang (1)
Zhiyi Zhang (1)
Yannan Liu (1) (3)
Xiaoping An (1)
Xiaolu Xu (4)
Yigang Tong (1)

1. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China
2. The State Key Laboratory of Medical Genetics and School of Life Sciences, Central South University, Changsha, 410078, China
3. The 307th Hospital of Chinese Peoples Liberation Army, Beijing, 100071, China
4. Beijing Aviation Meterological and Chemical Defense Research Institute, Beijing, 100071, China

刊物主题：Virology; Medical Microbiology; Oncology; Biochemistry, general; Microbiology; Microbial Genetics and Genomics;

出版者：Springer Berlin Heidelberg

ISSN：1995-820X

文摘

Methicillin-resistant Staphylococcus aureus (MRSA) is an increasing cause of serious infection, both in the community and hospital settings. Despite sophisticated strategies and efforts, the antibiotic options for treating MRSA infection are narrowing because of the limited number of newly developed antimicrobials. Here, four newly-isolated MRSA-virulent phages, IME-SA1, IMESA2, IME-SA118 and IME-SA119, were sequenced and analyzed. Their genome termini were identified using our previously proposed "termini analysis theory". We provide evidence that remarkable conserved terminus sequences are found in IME-SA1/2/118/119, and, moreover, are widespread throughout Twortlikevirus Staphylococcus phage G1 and K species. Results also suggested that each phage of the two species has conserved 5′ terminus while the 3′ terminus is variable. More importantly, a variable region with a specific pattern was found to be present near the conserved terminus of Twortlikevirus S. phage G1 species. The clone with the longest variable region had variable terminus lengths in successive generations, while the clones with the shortest variable region and with the average length variable region maintained the same terminal length as themselves during successive generations. IME-SA1 bacterial infection experiments showed that the variation is not derived from adaptation of the phage to different host strains. This is the first study of the conserved terminus and variable region of Twortlikevirus S. phages.