A universal strategy for high-yield production of soluble and functional clostridial collagenases in E. coli

详细信息    查看全文

• 作者：Paulina Ducka ; Ulrich Eckhard ; Esther Sch?nauer ; Stefan Kofler ; Gerhard Gottschalk ; Hans Brandstetter and Dorota N¨¹ss
• 关键词：Clostridial collagenases ; Expression ; Purification ; Platform
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2009
• 出版时间：July, 2009
• 年：2009
• 卷：83
• 期：6
• 页码：1055-1065
• 全文大小：636.0 KB

文摘

Clostridial collagenases are foe and friend: on the one hand, these enzymes enable host infiltration and colonization by pathogenic clostridia, and on the other hand, they are valuable biotechnological tools due to their capacity to degrade various types of collagen and gelatine. However, the demand for high-grade preparations exceeds supply due to their pathogenic origin and the intricate purification of homogeneous isoforms. We present the establishment of an Escherichia coli expression system for a variety of constructs of collagenase G (ColG) and H (ColH) from Clostridium histolyticum and collagenase T (ColT) from Clostridium tetani, mimicking the isoforms in vivo. Based on a setup of five different expression strains and two expression vectors, 12 different constructs were expressed, and a flexible purification platform was established, consisting of various orthogonal chromatography steps adaptable to the individual needs of the respective variant. This fast, cost-effective, and easy-to-establish platform enabled us to obtain at least 10 mg of highly pure mono-isoformic protein per liter of culture, ideally suited for numerous sophisticated downstream applications. This production and purification platform paves the way for systematic screenings of recombinant collagenases to enlighten the biochemical function and to identify key residues and motifs in collagenolysis.