Sustained effects of blue light on Streptococcus mutans in regrown biofilm

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• 作者：Julie Cohen-Berneron ; Doron Steinberg ; John D. B. Featherstone…
• 关键词：Acid production ; Caries ; Extracellular polysaccharide ; Virulence factors ; Visible light ; Antibacterial effect
• 刊名：Lasers in Medical Science
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：31
• 期：3
• 页码：445-452
• 全文大小：1,358 KB
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• 作者单位：Julie Cohen-Berneron (1) (3)
  Doron Steinberg (1)
  John D. B. Featherstone (2)
  Osnat Feuerstein (3)
  
  1. Biofilm Research Laboratory, Institute of Dental Sciences, Faculty of Dental Medicine, Hebrew University-Hadassah, Jerusalem, Israel
  3. Department of Prosthodontics, Faculty of Dental Medicine, Hebrew University-Hadassah, Jerusalem, Israel
  2. School of Dentistry, University of California San Francisco, San Francisco, CA, USA
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Medicine/Public Health, general
  Dentistry
  Laser Technology and Physics and Photonics
  Quantum Optics, Quantum Electronics and Nonlinear Optics
  Applied Optics, Optoelectronics and Optical Devices
  
• 出版者：Springer London
• ISSN：1435-604X

文摘

In prior studies, exposure of Streptococcus mutans in biofilm to blue light using high fluences of up to 680 J/cm2 did not interfere with bacterial capability to reform an initial biofilm; however, a delayed antibacterial effect was observed. Our aim was to determine the sustained effecttts of blue light-emitting diode (LED) curing light on the pathogenicity of the newly formed biofilm. S. mutans were grown to form biofilm that was exposed to blue light (wavelengths, 460–480 nm) for 1, 3, and 7 min (equivalent to 37, 112, and 262 J/cm2, respectively). Then, bacteria were suspended and allowed to regrow into new biofilms. The regrown biofilms were assessed for bacterial quantification by optical density (OD) measurement and quantitative polymerase chain reaction (qPCR), bacterial viability and extracellular polysaccharide production by fluorescent staining using confocal scanning laser microscopy, acid production by bacteria (acidogenicity), and bacterial survival at low pH (aciduricity) using qPCR. Bacterial growth in the regrown biofilms was increased when samples were previously exposed to light; however, under the confocal microscopy, a higher proportion of dead bacteria and a reduction in polysaccharide production were observed. The acidogenicity from the regrown biofilm was lowered as fluences of light increased. The aciduricity of the regrown biofilm was decreased, meaning less growth of bacteria into biofilm in low pH with increasing fluences. Blue light has sustained effects on S. mutans bacteria grown into new biofilm. Although bacterial growth in biofilm increased, bacterial viability and virulence characteristics were impaired. The cariogenic potential over time of S. mutans previously exposed to blue light when grown on tooth surfaces is yet to be determined.