Native structure of rat liver immune proteasomes

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• 作者：A. A. Stepanova ; Yu. V. Lyupina ; N. P. Sharova…
• 刊名：Doklady Biochemistry and Biophysics
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：468
• 期：1
• 页码：200-202
• 全文大小：396 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Biophysics and Biomedical Physics
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3091
• 卷排序：468

文摘

Native structure of active forms of rat liver immune proteasomes has been studied by two-dimensional electrophoresis method modified for analysis of unpurified protein fractions. The developed method allowed revealing the proteasome immune subunits LMP7 and LMP2 in 20S subparticles and in the structures bound to one or two PA28αβ activators, but not to the PA700 activator, which is involved in the hydrolysis of ubiquitinated proteins. The results obtained indicate the participation of the immune proteasomes in delicate regulatory mechanisms based on the production of biologically active peptides and exclude their participation in processes of crude degradation of “rotated” ubiquitinated proteins.Original Russian Text © A.A. Stepanova, Yu.V. Lyupina, N.P. Sharova, P.A. Erokhov, 2016, published in Doklady Akademii Nauk, 2016, Vol. 468, No. 3, pp. 339–341.Presented by Academician M.P. Kirpichnikov November 30, 2015