Comparison of tissue harvest protocols for the quantitation of acylcarnitines in mouse heart and liver by mass spectrometry
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- 作者：Christopher Petucci (1)
Stella Rojas-Betancourt (1)
Stephen J. Gardell (1) - 关键词：Acylcarnitines ; Tissue harvest ; Tissue processing ; Mass spectrometry
- 刊名：Metabolomics
- 出版年：2012
- 出版时间：October 2012
- 年：2012
- 卷：8
- 期：5
- 页码：784-792
- 全文大小：284KB
- 参考文献：1. 脕lvarez-S谩nchez, B., Priego-Capote, F., & Luque de Castro, M. D. (2010). Metabolomics analysis II. Preparation of biological samples prior to detection. / TrAC Trends in Analytical Chemistry, / 29, 120鈥?27. j.trac.2009.12.004">CrossRef
2. An, J., Muoio, D. M., Shiota, M., Fujimoto, Y., Cline, G. W., Shulman, G. I., et al. (2004). Hepatic expression of malonyl-CoA decarboxylase reverses muscle, liver and whole-animal insulin resistance. / Nature Medicine, / 10, 268鈥?74. CrossRef
3. Bain, J. R., Stevens, R. D., Wenner, B. R., Ilkayeva, O., Muoio, D. M., & Newgard, C. B. (2009). Metabolomics applied to diabetes research; Moving from information to knowledge. / Diabetes, / 58, 2429鈥?443. CrossRef
4. Beger, R. D., Sun, J., & Schnackenberg, L. K. (2010). Metabolomics approaches for discovering biomarkers of drug-induced hepatotoxicity and nephrotoxicity. / Toxicology and Applied Pharmacology, / 243, 154鈥?66. j.taap.2009.11.019">CrossRef
5. Brass, E. P., & Hoppel, C. L. (1981). / In vivo studies of carnitine and fatty acid metabolism: Problems with the use of anesthetics. / Analytical Biochemistry, / 110, 77鈥?1. CrossRef
6. Bremer, J. (1990). The role of carnitine in intracellular metabolism. / Journal of Clinical Chemistry and Clinical Biochemistry, / 28, 297鈥?01.
7. Bruce, S. J., Tavazzi, I., Parisod, V., Rezzi, S., Kochhar, S., & Guy, P. A. (2009). Investigation of human blood plasma sample preparation for performing metabolomics using ultrahigh performance liquid chromatography/mass spectrometry. / Analytical Chemistry, / 81, 3285鈥?296. CrossRef
8. Camina, M. F., Rozas, I., Gomez, M., Paz, J. M., Alonso, C., & Rodriguez-Segade, S. (1991). Short-term effects of administration of anticonvulsant drugs on free carnitine and acycarnitine in mouse serum and tissues. / British Journal of Pharmacology, / 103, 1179鈥?183. j.1476-5381.1991.tb12320.x">CrossRef
9. Chace, D. H., Hillman, S. L., van Hove, J. L. K., & Naylor, E. W. (1997). Rapid diagnosis of MCAD deficiency: Quantitative analysis of octanoylcarnitine and other acylcarnitines in newborn blood spots by tandem mass spectrometry. / Clinical Chemistry, / 43, 2106鈥?113.
10. Ford, D. A., Han, X., Horner, C. C., & Gross, R. W. (1996). Accumulation of unsaturated acylcarnitine molecular species during acute myocardial ischemia: Metabolic compartmentalization of produces of fatty acyl chain elongation in the acylcarnitine pool. / Biochemistry, / 35, 7903鈥?909. CrossRef
11. French, T. J., Goode, A. W., & Sugden, M. C. (1986). Ischaemia and tissue pyruvate activities in the rat: a comparison of the effects of cervical dislocation and pentobarbital anesthesia. / Biochemistry International, / 13, 843鈥?52.
12. Hirschey, M. D., Shimazu, T., Goetzman, E., Jing, E., Schwer, B., Lombard, D. B., et al. (2010). SIRT3 regulates mitochondrial fatty-acid oxidation by reversible enzyme deacetylation. / Nature, / 464, 121鈥?25. CrossRef
13. Jensen, M. V., Joseph, J. W., Olga Ilkayeva, O., Burgess, S., Lu, D., Ronnebaum, S. M., et al. (2006). Compensatory responses to pyruvate carboxylase suppression in Islet 尾-cells. Preservation of glucose-stimulated insulin secretion. / Journal of Biological Chemistry, / 281, 22342鈥?2351. jbc.M604350200">CrossRef
14. Kawaguchi, H., Hirakawa, K., Miyauchi, K., Koike, K., Ohno, Y., & Sakamoto, A. (2010). Pattern recognition analysis of proton nuclear magnetic resonance spectra of brain tissue extracts from rats anesthetized with propofol or Isoflurane. / PLoS One, / 5, e11172. journal.pone.0011172">CrossRef
15. Kien, C. L., Everingham, K. I., Stevens, R. D., Fukagawa, N. K., & Muoio, D. M. (2011). Short-term effects of dietary fatty acids on muscle lipid composition and serum acylcarnitine profile in human subjects. / Obesity, / 19, 305鈥?11. CrossRef
16. Koves, T. R., Ussher, J. R., Noland, R. C., Slentz, D., Mosedale, M., Ilkayeva, O., et al. (2008). Mitochondrial overload and incomplete fatty acid oxidation contribute to skeletal muscle insulin resistance. / Cell Metabolism, / 7, 45鈥?6. j.cmet.2007.10.013">CrossRef
17. Lin, C. Y., Wu, H., Tjeerdema, R. S., & Viant, M. R. (2007). Evaluation of metabolite extraction strategies from tissue samples using NMR metabolomics. / Metabolomics, / 3, 55鈥?7. CrossRef
18. Ljubkovic, M., Mio, Y., Marinovic, J., Stadnicka, A., Warltier, D. C., Bosnjak, Z. J., et al. (2007). Isoflurane preconditioning uncouples mitochondria and protects against hypoxia-reoxygenation. / American Journal of Physiology-Cell Physiology, / 292, C1583鈥揅1590. jpcell.00221.2006">CrossRef
19. Mancuso, D. J., Abendschein, D. R., Jenkins, C. M., Han, X., Saffitz, J. E., Schuessler, R. B., et al. (2003). Cardiac ischemia activates calcium-independent phospholipase A2尾, precipitating ventricular tachyarrhythmias in transgenic mice. / Journal of Biological Chemistry, / 278, 22231鈥?2236. jbc.C300033200">CrossRef
20. Masson, P., Alves, A. C., Ebbels, T. M. D., Nicholson, J. K., & Want, E. J. (2010). Optimization and evaluation of metabolite extraction protocols for untargeted metabolic profiling of liver samples by UPLC-MS. / Analytical Chemistry, / 82, 7779鈥?786. CrossRef
21. Mendrick, D. L., & Schnackenberg, L. (2009). Genomic and metabolomics advances in the identification of disease and adverse event biomarkers. / Biomarkers in Medicine, / 3, 605鈥?15. CrossRef
22. Millington, D. S., Kodo, N., Norwood, D. L., & Roe, C. R. (1990). Tandem mass spectrometry: A new method for acycarntine profling with potential for neonatal screening for inborn errors of metabolism. / Journal of Inherited Metabolic Disease, / 13, 321鈥?24. CrossRef
23. Newgard, C. B., An, J., Bain, J. R., Muehlbauer, M. J., Stevens, R. D., Lien, L. F., et al. (2009). Metabolic signature that differentiates obese and lean humans and contributes to insulin resistance. / Cell Metabolism, / 9, 311鈥?26. j.cmet.2009.02.002">CrossRef
24. Nicholson, J. K., & Lindon, J. C. (2008). Metabonomics. / Nature, / 455, 1054鈥?056. CrossRef
25. Park, E.-S., Daily, J. W., & Sachan, D. S. (2001). Anesthetics and cardiocentesis increased urinary carnitine excretion in rats and guinea pigs. / Nutrition Research, / 21, 531鈥?40. CrossRef
26. Ramsay, R. R., & Zammit, V. A. (2004). Carnitine acyltransferases and their influence on CoA pools in health and disease. / Molecular Aspects of Medicine, / 25, 475鈥?93. j.mam.2004.06.002">CrossRef
27. Robertson, D. G., Reily, M. D., & Baker, D. (2007). Metabonomics in pharmaceutical discovery and development. / Journal of Proteome Research, / 6, 526鈥?39. CrossRef
28. Sedlic, F., Pravdic, D., Hirata, N., Mio, Y., Sepac, A., Camara, A. K., et al. (2010). Monitoring mitochondrial electron fluxes using NAD(P)H-flavoprotein fluormetry reveals complex action of isoflurane on cardiomyocytes. / Biochimica et Biophysica Acta, / 1797, 1749鈥?758. j.bbabio.2010.07.009">CrossRef
29. Seifert, E. L., Fiehn, O., Bezaire, V., Bickel, D. R., Wohlgemuth, G., Adams, S. H., et al. (2010). Long-chain fatty acid combustion rate is associated with unique metabolite profiles in skeletal muscle mitochondria. / PLoS One, / 5, e9834. journal.pone.0009834">CrossRef
30. Sewell, A. C., & B枚hles, H. J. (1995). Acylcarnitines in intermediary metabolism. / European Journal of Pediatrics, / 154, 871鈥?77. CrossRef
31. Son, N. H., Yu, S., Tuinei, J., Arai, K., Hamai, H., Homma, S., et al. (2010). PPAR纬-induced cardiolipotoxicity in mice is ameliorated by PPAR伪 deficiency despite increases in fatty acid oxidation. / Journal of Clinical Investigation, / 120, 3443鈥?454. CrossRef
32. Sreekumar, A., Poisson, L. M., Rajendiran, T. M., Khan, A. P., Cao, Q., Yu, J., et al. (2009). Metabolomic profiles delineate potential role for sarcosine in prostate cancer progression. / Nature, / 457, 910鈥?14. CrossRef
33. Su, X., Han, X., Mancuso, D. J., Abendschein, D. R., & Gross, R. W. (2005). Accumulation of long-chain acylcarnitine and 3-hydroxy acylcarnitine molecular species in diabetic myocardium: Identification of alterations in mitochondrial fatty acid processing in diabetic myocardium by shotgun lipidomics. / Biochemistry, / 44, 5234鈥?245. CrossRef
34. Turer, A. T., Stevens, R. D., Bain, J. R., Muehlbauer, M. J., van der Westhuizen, J., Mathew, J. P., et al. (2009). Metabolomic profiling reveals distinct patterns of myocardial substrate use in humans with coronary artery disease or left ventricular dysfunction during surgical ischemia/reperfusion. / Circulation, / 119, 1736鈥?746. CrossRef
35. Van Vlies, N., Tan, L., Overmars, H., Bootsma, A. H., Kulik, W., Wanders, R. J. A., et al. (2005). Characterization of carnitine and fatty acid metabolism in the long-chain acyl-CoA dehydrogenase-deficient mouse. / Biochemical Journal, / 387, 185鈥?93. CrossRef
36. Wang, L., Ko, K. W. S., Lucchinetti, E., Zhang, L., Troxler, H., Hersberger, M., et al. (2010). Metabolic profiling of hearts exposed to sevoflurane and propofol reveals distinct regulation of fatty acid and glucose oxidation: CD36 and pyruvate dehydrogenase as key regulators in anesthetic-induced fuel shift. / Anesthesiology, / 113, 541鈥?51.
37. Yanes, O., Clark, J., Wong, D. M., Patti, G. J., S谩nchez-Ruiz, A., Benton, H. P., et al. (2010). Metabolic oxidation regulates embryonic stem cell differentiation. / Nature Chemical Biology, / 6, 411鈥?17. CrossRef - 作者单位：Christopher Petucci (1)
Stella Rojas-Betancourt (1)
Stephen J. Gardell (1)
1. Metabolomics Core, Sanford-Burnham Medical Research Institute, 6400 Sanger Rd., Orlando, FL, 32827, USA - ISSN：1573-3890
文摘
Profiling of acylcarnitines (ACs) in tissues and biological fluids by mass spectrometry is a powerful approach to examine the impact of genetic, pharmacological, and environmental factors on intermediary metabolism. The AC pool exhibits rapid changes in composition and abundance in response to altered cellular fuel catabolism. However, the mercurial nature of the AC pool makes it prone to spurious influences arising from experimental variables related to tissue harvesting and processing. We evaluated the impact of various strategies to anesthetize, sedate, or euthanize (A/S/E) mice which included Nembutal, Beuthanasia, isoflurane, ketamine/xylazine (Ket/Xy) and CO2/cervical dislocation (CD) on the tissue AC profiles. ACs extracted from heart and liver were derivatized to methyl esters and quantitated by mass spectrometry. Marked differences were seen in the tissue AC profiles, especially in heart, depending upon the choice of the A/S/E strategy. Most importantly, a uniform A/S/E protocol must be employed. While tissue AC profiles in situ cannot be unambiguously defined, use of Nembutal appears to be superior to other A/S/E strategies especially when assessing the AC levels in the heart. We also showed that it is preferable to expeditiously harvest and flash-freeze tissues to avoid procedure-related perturbation of the AC profile. A more protracted tissue harvest, when recovering numerous tissues from the same animal, can alter the AC pool. In conclusion, this investigation provides key guidance for harvesting heart and liver from mice in order to minimize the procedure-associated change of the AC pool which can mask the influence of the intended experimental variables.