Nuclear and mitochondrial genes for inferring Trichuris phylogeny

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• 作者：Rocío Callejón ; Cristina Cutillas ; Steven A. Nadler
• 关键词：Trichuris ; Molecular phylogeny ; Triose phosphate isomerase ; cox1 mtDNA ; cob mtDNA ; 18S rRNA
• 刊名：Parasitology Research
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：114
• 期：12
• 页码：4591-4599
• 全文大小：495 KB
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• 作者单位：Rocío Callejón (1)
  Cristina Cutillas (1)
  Steven A. Nadler (2)
  
  1. Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, 41012, Seville, Spain
  2. Department of Entomology and Nematology, University of California, Davis, CA, 95616, USA
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Microbiology
  Immunology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1955

文摘

Nucleotide sequences of the triose phosphate isomerase (TPI) gene (624 bp) and mitochondrial cytochrome b (cob) gene (520 bp) were obtained by PCR and evaluated for utility in inferring the phylogenetic relationships among Trichuris species. Published sequences of one other nuclear gene (18S or SSU rRNA, 1816-846 bp) and one additional mitochondrial (mtDNA) gene (cytochrome oxidase 1, cox1, 342 bp) were also analyzed. Maximum likelihood and Bayesian inference methods were used to infer phylogenies for each gene separately but also for the combined mitochondrial data (two genes), the combined nuclear data (two genes), and the total evidence (four gene) dataset. Few Trichuris clades were uniformly resolved across separate analyses of individual genes. For the mtDNA, the cob gene trees had greater phylogenetic resolution and tended to have higher support values than the cox1 analyses. For nuclear genes, the SSU gene trees had slightly greater resolution and support values than the TPI analyses, but TPI was the only gene with reliable support for the deepest nodes in the tree. Combined analyses of genes yielded strongly supported clades in most cases, with the exception of the relationship among Trichuris clades 1, 2, and 3, which showed conflicting results between nuclear and mitochondrial genes. Both the TPI and cob genes proved valuable for inferring Trichuris relationships, with greatest resolution and support values achieved through combined analysis of multiple genes. Based on the phylogeny of the combined analysis of nuclear and mitochondrial genes, parsimony mapping of definitive host utilization depicts artiodactyls as the ancestral hosts for these Trichuris, with host-shifts into primates, rodents, and Carnivora. Keywords Trichuris Molecular phylogeny Triose phosphate isomerase cox1 mtDNA cob mtDNA 18S rRNA