Prion strains are differentially released through the exosomal pathway

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• 作者：Zaira E. Arellano-Anaya ; Alvina Huor…
• 关键词：Prion release ; Cell models ; Exosome ; Extracellular vesicle ; Prion spiking ; TSE agent
• 刊名：Cellular and Molecular Life Sciences (CMLS)
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：72
• 期：6
• 页码：1185-1196
• 全文大小：1,368 KB
• 参考文献：1. Collinge, J (2001) Prion diseases of humans and animals: their causes and molecular basis. Annu Rev Neurosci 24: pp. 519-550 CrossRef
  2. Prusiner, SB (1998) Prions. Proc Natl Acad Sci USA 95: pp. 13363-13383 CrossRef
  3. Castilla, J, Saa, P, Hetz, C, Soto, C (2005) In vitro generation of infectious scrapie prions. Cell 121: pp. 195-206 CrossRef
  4. Beringue, V, Vilotte, JL, Laude, H (2008) Prion agent diversity and species barrier. Vet Res 39: pp. 47 CrossRef
  5. Mabbott, NA, MacPherson, GG (2006) Prions and their lethal journey to the brain. Nat Rev Microbiol 4: pp. 201-211 CrossRef
  6. Vilette, D (2008) Cell models of prion infection. Vet Res 39: pp. 10 CrossRef
  7. Kanu, N, Imokawa, Y, Drechsel, DN, Williamson, RA, Birkett, CR (2002) Transfer of scrapie prion infectivity by cell contact in culture. Curr Biol 12: pp. 523-530 CrossRef
  8. Paquet, S, Langevin, C, Chapuis, J, Jackson, GS, Laude, H (2007) Efficient dissemination of prions through preferential transmission to nearby cells. J Gen Virol 88: pp. 706-713 CrossRef
  9. Langevin, C, Gousset, K, Costanzo, M, Richard-Le Goff, O, Zurzolo, C (2010) Characterization of the role of dendritic cells in prion transfer to primary neurons. Biochem J 431: pp. 189-198 CrossRef
  10. Gousset, K, Schiff, E, Langevin, C, Marijanovic, Z, Caputo, A (2009) Prions hijack tunnelling nanotubes for intercellular spread. Nat Cell Biol 11: pp. 328-336 CrossRef
  11. Mattei, V, Barenco, MG, Tasciotti, V, Garofalo, T, Longo, A (2009) Paracrine diffusion of PrP(C) and propagation of prion infectivity by plasma membrane-derived microvesicles. PLoS ONE 4: pp. e5057 CrossRef
  12. Alais, S, Simoes, S, Baas, D, Lehmann, S, Raposo, G (2008) Mouse neuroblastoma cells release prion infectivity associated with exosomal vesicles. Biol Cell 100: pp. 603-615 CrossRef
  13. Fevrier, B, Vilette, D, Archer, F, Loew, D, Faigle, W (2004) Cells release prions in association with exosomes. Proc Natl Acad Sci U S A 101: pp. 9683-9688 CrossRef
  14. Vella, LJ, Sharples, RA, Lawson, VA, Masters, CL, Cappai, R (2007) Packaging of prions into exosomes is associated with a novel pathway of PrP processing. J Pathol 211: pp. 582-590 CrossRef
  15. Bellingham, SA, Guo, BB, Coleman, BM, Hill, AF (2012) Exosomes: vehicles for the transfer of toxic proteins associated with neurodegenerative diseases?. Front Physiol 3: pp. 124 CrossRef
  16. Schneider, A, Simons, M (2013) Exosomes: vesicular carriers for intercellular communication in neurodegenerative disorders. Cell Tissue Res 352: pp. 33-47 CrossRef
  17. Vingtdeux, V, Sergeant, N, Buee, L (2012) Potential contribution of exosomes to the prion-like propagation of lesions in Alzheimer’s disease. Front Physiol 3: pp. 229 CrossRef
  18. Arellano-Anaya, ZE, Savistchenko, J, Mathey, J, Huor, A, Lacroux, C (2011) A simple, versatile and sensitive cell-based assay for prions from various species. PLoS ONE
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Biomedicine
  Life Sciences
  Biochemistry
  
• 出版者：Birkh盲user Basel
• ISSN：1420-9071

文摘

Cell-to-cell transfer of prions is a crucial step in the spreading of prion infection through infected tissue. At the cellular level, several distinct pathways including direct cell–cell contacts and release of various types of infectious extracellular vesicles have been described that may potentially lead to infection of na?ve cells. The relative contribution of these pathways and whether they may vary depending on the prion strain and/or on the infected cell type are not yet known. In this study we used a single cell type (RK13) infected with three different prion strains. We showed that in each case, most of the extracellular prions resulted from active cell secretion through the exosomal pathway. Further, quantitative analysis of secreted infectivity indicated that the proportion of prions eventually secreted was dramatically dependent on the prion strain. Our data also highlight that infectious exosomes secreted from cultured cells might represent a biologically pertinent material for spiking experiments. Also discussed is the appealing possibility that abnormal PrP from different prion strains may differentially interact with the cellular machinery to promote secretion.