Wohlfahrtiimonas larvae sp. nov., isolated from the larval gut of Hermetia illucens (Diptera: Stratiomyidae)

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• 作者：Jae Kook Lee (1)
  Youn Yeop Lee (1)
  Kwan Ho Park (2)
  Jeonggu Sim (3)
  Youngcheol Choi (2)
  Sung-Jae Lee (1)
  
• 关键词：Wohlfahrtiimonas larvae ; Hermetia illucens ; Larval gut microorganism ; Polyphasic taxonomy
• 刊名：Antonie van Leeuwenhoek
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：105
• 期：1
• 页码：15-21
• 全文大小：456 KB
• 作者单位：Jae Kook Lee (1)
  Youn Yeop Lee (1)
  Kwan Ho Park (2)
  Jeonggu Sim (3)
  Youngcheol Choi (2)
  Sung-Jae Lee (1)
  
  1. Department of Biology, Kyung Hee University, Seoul, 130-701, South Korea
  2. Insect Resources Division, Department of Agricultural Biology, National Academy of Agricultural Science and Technology, Rural Development Administration, Suwon, 441-853, South Korea
  3. Department of Visual Optics, Baekseok University, Cheonan, 330-704, South Korea
  
• ISSN：1572-9699

文摘

A novel, Gram-negative, facultative anaerobic, motile and short rod-shaped bacterium, strain KBL006T was isolated from the larval gut of Hermetia illucens, Black soldier fly. The 16S rRNA gene sequence of strain KBL006T showed 96.4?% similarity to that of Wohlfahrtiimonas chitiniclastica S5T. Strain KBL006T grew optimally at 30?°C, at pH 8.0 and in the presence of 1-?% (w/v) NaCl. Oxidase activity and catalase activity were positive. The major fatty acids were C18:1 ω7c, C14:0, and C16:0. The major respiratory quinone was ubiquinone-8 (Q-8). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol, and two phospholipids. The G+C content of the genomic DNA was 45.2?mol%. Based on these polyphasic data, strain KBL006T is considered to represent a novel species in the genus Wohlfahrtiimonas, for which the name Wohlfahrtiimonas larvae sp. nov. is proposed. The type strain is KBL006T (=?KACC 16839T?=?JCM 18424T).