Chitosan as a potential osteogenic factor compared with dexamethasone in cultured macaque dental pulp stromal cells
详细信息 查看全文
- 作者：Lisa R. Amir (1)
Dewi F. Suniarti (1)
Sri Utami (1)
Basril Abbas (2) - 关键词：Chitosan ; Dexamethasone ; Dental pulp stromal cells ; Osteogenic supplement ; Macaque
- 刊名：Cell and Tissue Research
- 出版年：2014
- 出版时间：November 2014
- 年：2014
- 卷：358
- 期：2
- 页码：407-415
- 全文大小：800 KB
- 参考文献：1. Amaral IF, Lamghari M, Sousa SR, Sampaio P, Barbosa MA (2005) Rat bone marrow stromal cells osteogenic differentiation and fibronectin adsorption on chitosan membranes: the effect of the degree of acetylation. J Biomed Mater Res Part A 75A:387-97 CrossRef
2. Amaral IF, Sampaio P, Barbosa MA (2006) Three-dimensional culture of human osteoblastic cells in chitosan sponges: the effect of the degree of acetylation. J Biomed Mater Res 76:335-46 CrossRef
3. Ambre AH, Katti DR, Katti KS (2013) Nanoclays mediate stem cell differentiation and mineralized ECM formation on biopolymer scaffolds. J Biomed Mater Res Part A 101:2644-660 CrossRef
4. Cai K, Hu Y, Jandt KD, Wang Y (2008) Surface modification of titanium thin film with chitosan via electrostatic self-assembly technique and its influence on osteoblast growth behavior. J Mater Sci Mater Med 19:499-06 CrossRef
5. Casta?eda F, Kinne RK (2000) Cytotoxicity of millimolar concentrations of ethanol on HepG2 human tumor cell line compared to normal rat hepatocytes in vitro. J Cancer Res Clin Oncol 126:503-10 CrossRef
6. Chadipiralla K, Yochim JM, Bahuleyan B, Huang CY, Garcia-Godoy F, Murray PE, Stelnicki EJ (2010) Osteogenic differentiation of stem cells derived from human periodontal ligaments and pulp of human exfoliated deciduous teeth. Cell Tissue Res 340:323-33 CrossRef
7. Cheng SL, Yang JW, RIfas L, Zhang SF, Avioli LV (1994) Differentiation of human bone marrow osteogenic stromal cells in vitro: induction of the osteoblast phenotype by dexamethasone. Endocrinology 134:277-86
8. Cho JH, Kim SH, Park KD, Jung MC, Yang WI, Han SW, Noh JY, Lee JW (2004) Chondrogenic differentiation of human mesenchymal stem cells using a thermosensitive poly (N-isopropylacrylamide) and water-soluble chitosan copolymer. Biomaterials 25:5743-751 CrossRef
9. Geesin JC, Hendricks LJ, Gordon JS, Berg RA (1991) Modulation of collagen synthesis by growth factors: the role of ascorbate-stimulated lipid peroxidation. Arch Biochem Biophys 289:6-1 CrossRef
10. Gharibi B, Hughes FJ (2012) Effect of medium supplements on proliferation, differentiation potential and in vitro expansion of mesenchymal stem cells. Stem Cell Trans Med 1:771-82 CrossRef
11. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S (2000) Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A 97:13625-3630 CrossRef
12. Guzmán-Morales J, El-Gabalawy H, Pham MH, Tran-Khanh N, McKee MD, Wu W, Centola M, Hoemann CD (2009) Effect of chitosan particles and dexamethasone on human bone marrow stromal cell osteogenesis and angiogenic factor secretion. Bone 45:617-26 CrossRef
13. Hamidouche Z, Hay E, Vaudin P, Charbord P, Schule R, Marie PJ, Fromigue O (2008) FHL2 mediates dexamethasone-induced mesenchymal cell differentiation into osteoblasts by activating Wnt/β-catenin signaling-dependent Runx2 expression. FASEB J 22:3813-822 CrossRef
14. Hoemann CD, Sun J, Legare A, McKee MD, Buschmann MD (2005) Tissue engineering of cartilage using an injectable and adhesive chitosan-based cell-delivery vehicle. Osteoarthritis Cartilage 13:318-29 CrossRef
15. Hoemann CD, Sun J, McKee MD, Chevrier A, Rossomacha E, Rivard GE, Hurtig M, Buschmann MD (2007) Chitosan-glycerol phosphate/blood implants elicit hyaline cartilage repair integrated with porous subchondral bone in microdrilled rabbit - 作者单位：Lisa R. Amir (1)
Dewi F. Suniarti (1)
Sri Utami (1)
Basril Abbas (2)
1. Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Salemba Raya No.4, Jakarta Pusat, 10430, Indonesia
2. Center for Application of Isotope and Radiation Technology, National Atomic Energy Agency, Jakarta, Indonesia - ISSN：1432-0878
文摘
Chitosan, a natural biopolymer derived from chitin, is considered a promising scaffold material for bone tissue engineering. The ability of chitosan to promote the osteogenic differentiation of dental pulp stromal/stem cells (DPSCs) is unknown. We have evaluated the potential of chitosan to induce the osteogenic differentiation of macaque DPSCs in comparison with that of dexamethasone. DPSCs were cultured in mineralizing medium supplemented with 5 or 10?μg/ml chitosan or with 1 or 10 nM dexamethasone. The metabolic activity of DPSCs was measured by MTT assay. Their osteogenic differentiation was determined by the number of transcripts of RUNX2, alkaline phosphatase (ALP), and COL1A1 by using real-time polymerase chain reaction, by alizarin red staining for mineral deposition, and by the ALP activity released into the medium for their ability to support biomineralizaton. Addition of chitosan to the mineralizing medium significantly increased DPSCs metabolism after 7 and 14?days of culture (P?≤-.0001). Chitosan at 5?μg/ml also significantly enhanced RUNX2 and ALP mRNA but not COL1A1 mRNA; chitosan tended to increase the release of ALP hydrolytic enzyme activity into the medium during the first week. Dexamethasone upregulated the osteogenic markers tested. Mineral deposition was similar in the chitosan and dexamethasone groups and was not statistically different from that of the mineralizing control group. Thus, the potential of chitosan to stimulate DPSCs proliferation and early osteogenic differentiation is comparable with that of dexamethasone, but mineralization remains unaffected by chitosan treatment. In addition to its role as a three-dimensional scaffold for osteogenic cells in vivo, chitosan might also stimulate DPSCs proliferation and early osteogenic differentiation in vitro.