Expression and distribution of grp-78/bip in mineralizing tissues and mesenchymal cells

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• 作者：Sriram Ravindran (1)
  Qi Gao (1)
  Amsaveni Ramachandran (1)
  Premanand Sundivakkam (2)
  Chinnaswamy Tiruppathi (2)
  Anne George (1) anneg@uic.edu
• 关键词：GRP ; 78 – ; DMP1 – ; Mineralization – ; Extracellular matrix – ; Bone – ; Dentin
• 刊名：Histochemistry and Cell Biology
• 出版年：2012
• 出版时间：July 2012
• 年：2012
• 卷：138
• 期：1
• 页码：113-125
• 全文大小：1.8 MB
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• 作者单位：1. Brodie Tooth Development Genetics and Regenerative Medicine Research Laboratory, Department of Oral Biology, University of Illinois at Chicago, Chicago, IL 60612, USA2. Department of Pharmacology, University of Illinois at Chicago, Chicago, IL 60612, USA
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Anatomy
  Medicine/Public Health, general
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-119X

文摘

Glucose-regulated protein 78 (GRP-78) is one of the many endoplasmic reticulum chaperone proteins that have been shown to possess multifunctional roles. We have previously demonstrated that GRP-78 functions as a receptor for dentin matrix protein 1 (DMP1) and is required for DMP1-mediated calcium release; that it is a secreted protein and can bind to type I collagen and DMP1 extracellularly and aid in the nucleation of calcium phosphate. We provide evidence in this study that tyrosine phosphorylation is required for DMP1/GRP-78-mediated calcium release in mesenchymal cells. We further demonstrate that GRP-78 is localized in the nucleus of mesenchymal cells and that the cell surface GRP-78 is not associated with the G-protein Gαq in mesenchymal cells. Results from this study show that during development of mineralized tissues, increased expression of GRP-78 can be observed in condensing cartilage and mesenchymal cells of the alveolar bone, endochondral bone and dental pulp. Additionally, we show that GRP-78 is present in the mineralizing matrices of teeth, bone and in the extracellular matrix of differentiating human marrow stromal cells and dental pulp stem cells. Collectively, our observations provide a new perspective on GRP-78 with respect to mineralized matrix formation.