The ESFRI Instruct Core Centre Frankfurt: automated high-throughput crystallization suited for membrane proteins and more

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• 作者：Yvonne Thielmann (1)
  Juergen Koepke (1)
  Hartmut Michel (1)
  
• 关键词：Calorimetry ; Crystallization ; High ; throughput ; Mass spectrometry ; Membrane protein ; X ; ray diffraction
• 刊名：Journal of Structural and Functional Genomics
• 出版年：2012
• 出版时间：June 2012
• 年：2012
• 卷：13
• 期：2
• 页码：63-69
• 全文大小：286KB
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• 作者单位：Yvonne Thielmann (1)
  Juergen Koepke (1)
  Hartmut Michel (1)
  
  1. Department of Molecular Membrane Biology, Max-Planck-Institute of Biophysics, Max-von-Laue Str. 3, 60438, Frankfurt, Germany
  

文摘

Structure determination of membrane proteins and membrane protein complexes is still a very challenging field. To facilitate the work on membrane proteins the Core Centre follows a strategy that comprises four labs of protein analytics and crystal handling, covering mass spectrometry, calorimetry, crystallization and X-ray diffraction. This general workflow is presented and a capacity of 20% of the operating time of all systems is provided to the European structural biology community within the ESFRI Instruct program. A description of the crystallization service offered at the Core Centre is given with detailed information on screening strategy, screens used and changes to adapt high throughput for membrane proteins. Our aim is to constantly develop the Core Centre towards the usage of more efficient methods. This strategy might also include the ability to automate all steps from crystallization trials to crystal screening; here we look ahead how this aim might be realized at the Core Centre.