文摘
From a genomic library enriched for AG repeats, 23 polymorphic microsatellite loci were isolated for Styrax confusus and Styrax japonicus. All the loci are polymorphic in Styrax japonicus. Analysis of 23 individuals from two populations revealed an average of 5.3 alleles per locus (range: 2-0), an average observed heterozygosity of 0.30 (range: 0-.826) and an average expected heterozygosity of 0.66 (range: 0.198-.888). Fourteen loci are polymorphic in Styrax confusus, showing 4-1 alleles per locus, an observed heterozygosity ranging from 0.087 to 0.870 and an expected heterozygosity ranging from 0.580 to 0.907. These polymorphic microsatellite loci provide useful molecular tools to study genetic variation and phylogeography of the two Styrax species across their ranges in eastern Asia.