A novel isolated Terrabacter-like bacterium can mineralize 2-aminopyrimidine, the principal metabolite of microbial sulfadiazine degradation

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• 作者：Wolfgang Tappe ; Diana Hofmann ; Ulrich Disko ; Stephan Koeppchen…
• 关键词：Sulfonamides ; Sulfadiazine ; 2 ; Aminopyrimidine ; Degradation ; Metabolite ; Mineralization
• 刊名：Biodegradation
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：26
• 期：2
• 页码：139-150
• 全文大小：852 KB
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  2. Bouju, H, Ricken, B, Beffa, T, Corvini, PFX, Kolvenbach, BA (2012) Isolation of bacterial strains capable of sulfamethoxazole mineralization from an acclimated membrane bioreactor. Appl Environ Microbiol 78: pp. 277-279 CrossRef
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  7. Habe, H, Chung, JS, Ishida, A, Kasuga, K, Die, K, Takemura, T, Nojiri, H, Yamane, H, Omori, T (2005) The fluorine catabolic linear plasmid in Terrabacter sp. strain DBF63 carries the ?-ketoadipate pathway genes, pcaRHGBDCFIJ, also found in proteobacteria. Microbiology 151: pp. 3713-3722 CrossRef
  8. Hartig C, (2000) Analytik, Vorkommen und Verhalten aromatischer Sulfonamide in der aquatischen Umwelt. Doctoral Dissertation, Technical University of Berlin, http://webdoc.sub.gwdg.de/ebook/diss/2003/tu-berlin/diss/2000/hartig_claudia.pdf
  9. Heldal, M, Norland, S, Tumyr, O (1985) X-ray microanalytic method for measurement of dry matter and elemental content of individual bacteria. Appl Environ Microbiol 50: pp. 1251-1257
  10. Hoffmann, T, Hofmann, D, Klumpp, E, Küppers, S (2011) Electrochemistry-mass spectrometry for mechanistic studies and simulations of oxidation processes in the environment. Anal Bioanal Chem 399: pp. 1859-1868 CrossRef
  11. Iida, T, Mukouzaka, Y, Nakamura, K, Kudo, T (2002) Plasmid-borne genes code for an angular dioxygenase involved in dibenzofuran degradation by Terrabacter sp. strain YK3. Appl Environ Microbiol 68: pp. 3716-3723 CrossRef
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  14. Kreuzig, R, H?ltge, S (2005) Investigations on the fate of sulfadiazine in manured soil: laboratory experiments and test plot studies. Environ Toxicol Chem 24: pp. 771-776 CrossRef
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• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment
  Soil Science and Conservation
  Geochemistry
  Terrestrial Pollution
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  Waste Management and Waste Technology
  
• 出版者：Springer Netherlands
• ISSN：1572-9729

文摘

Recently we showed that during the degradation of sulfadiazine (SDZ) by Microbacterium lacus strain SDZm4 the principal metabolite 2-aminopyrimidine (2-AP) accumulated to the same molar amount in the culture as SDZ disappeared (Tappe et al. Appl Environ Microbiol 79:2572-577, 2013). Although 2-AP is considered a recalcitrant agent, long-term lysimeter experiments with 14C-pyrimidine labeled SDZ ([14C]pyrSDZ) provided indications for substantial degradation of the pyrimidine moiety of the SDZ molecule. Therefore, we aimed to enrich 2-AP degrading bacteria and isolated a pure culture of a Terrabacter-like bacterium, denoted strain 2APm3. When provided with 14C-labeled SDZ, M. lacus strain SDZm4 degraded [14C]pyrSDZ to [14C]2-AP. Resting cells of 2APm3 at a concentration of 5?×?106?cells?ml? degraded 62?μM [14C]2-AP to below the detection limit (0.6?μM) within 5?days. Disappearance of 2-AP resulted in the production of at least two transformation products (M1 and M2) with M2 being identified as 2-amino-4-hydroxypyrimidine. After 36?days, the transformation products disappeared and 83?% of the applied [14C]2-AP radioactivity was trapped as 14CO2. From this we conclude that a consortium of two species should be able to almost completely degrade SDZ in soils.