Histopathological evaluation of the healing effects of human amniotic membrane transplantation in third-degree burn wound injuries

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• 作者：Anahita Sedighi ; Davood Mehrabani ; Reza Shirazi
• 关键词：Burn ; Wound healing ; Amniotic membrane ; Silver sulfadiazine
• 刊名：Comparative Clinical Pathology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：25
• 期：2
• 页码：381-385
• 全文大小：718 KB
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• 作者单位：Anahita Sedighi (1) (5)
  Davood Mehrabani (2)
  Reza Shirazi (3) (4)
  
  1. Cellular and Molecular Biology Department, Pharmaceutical Science Branch, Islamic Azad University, Tehran, Iran
  5. MSc of Cellular and Molecular Biology, Stem Cells and Transgenic Technology Research Center, Shiraz University of Medical Sciences, P.O. Box: 14336, 71348, Shiraz, Iran
  2. Stem Cells and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  3. Department of Anatomical Sciences, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Pathology
  Hematology
  Oncology
  
• 出版者：Springer London
• ISSN：1618-565X

文摘

Skin burn injuries result in loss of its protective function as a barrier and leading to a high risk of infection. Therefore, effective treatments and healing of burn injuries are very important to prevent complications. Amniotic membrane (AM) as a biological dressing inhibits the loss of vital fluids, water, and protein. The aim of this study was to compare the healing effects of AM and silver sulfadiazine (SSD) ointment in third-degree burn injuries in experimental rat model. Fifty-four male Sprague–Dawley rats were divided randomly into three equal groups. After induction of third-degree burn, transplantation of human AM (HAM) and SSD ointment used on wound area for treatment groups. The third group was considered as control. At days 7, 14, and 21, histopathological evaluation of burn wound area was performed using light microscopy. After 21 days, burn injury in HAM group showing lack of enough wound contraction and decrease in wound area in comparison to SSD group. Also, the healing effects were demonstrated using decline of inflammatory cell infiltration and enhanced epithelium after 21 days. The total wound score was significantly higher in the HAM group than the control and SSD ointment groups, during and at the end of the experiment (P < 0.05). On day 21, significantly lesser inflammatory cell infiltration was noticed in the control group (P < 0.05). Our findings showed that HAM can be used successfully as a biological treatment for experimental third-degree burn injury in animal model.