DNA barcoding supports identification of Malacobdella species (Nemertea: Hoplonemertea)
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- 作者：Jose E F Alfaya (1) (2)
Gregorio Bigatti (1) (2)
Hiroshi Kajihara (3)
Malin Strand (4)
Per Sundberg (5)
Annie Machordom (6)
1. LARBIM ; IBIOMAR- Centro Nacional Patag贸nico (CENPAT) ; CONICET ; Bvd. Brown 2915 ; U9120ACV ; Puerto Madryn ; Chubut ; Argentina
2. Facultad de Ciencias Naturales ; Universidad Nacional de la Patagonia San Juan Bosco (UNPSJB) ; Bvd. Brown S/N ; U9120ACV ; Puerto Madryn ; Chubut ; Argentina
3. Faculty of Science ; Hokkaido University ; Kita 10 ; Nishi 8 ; Kita-ku ; Sapporo ; 060-0810 ; Japan
4. The Swedish Species Information Centre ; SLU ; B盲ckl枚sav盲gen 10 ; Box 7007 ; SE-750 07 ; Uppsala ; Sweden
5. Department of Biological and Environmental Sciences ; University of Gothenburg ; Medicinaregatan 18 ; PO Box 463 ; SE-40530 ; Gothenburg ; Sweden
6. Museo Nacional de Ciencias Naturales (MNCN) ; CSIC ; Jos茅 Guti茅rrez Abascal ; 2 ; 28006 ; Madrid ; Spain - 关键词：DNA barcoding ; COI gene ; Bivalvia ; Entocommensal nemertean ; Malacobdella
- 刊名：Zoological Studies
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：54
- 期：1
- 全文大小：3,449 KB
- 参考文献：1. Alfaya JEF, Bigatti G, Machordom A (2013) Mitochondrial and nuclear markers reveal a lack of genetic structure in the entocommensal nemertean / Malacobdella arrokeana in the Patagonian gulfs. Helgoland Mar Res 67(2):407鈥?12
2. Andrade SCS, Strand M, Schwartz M, Chen H, Kajihara H, von D枚hren J, Sun S, Junoy J, Thiel M, Norenburg JL, Turbeville JM, Giribet G, Sundberg P (2012) Disentangling ribbon worm relationships: multi-locus analysis supports traditional classification of the phylum Nemertea. Cladistics 28:141鈥?59 class="external" href="http://dx.doi.org/10.1111/j.1096-0031.2011.00376.x" target="_blank" title="It opens in new window">CrossRef
3. Bucklin A, Steinke D, Blanco-Bercial L (2011) DNA barcoding of marine metazoa. Annu Rev Mar Sci 3:471鈥?08 class="external" href="http://dx.doi.org/10.1146/annurev-marine-120308-080950" target="_blank" title="It opens in new window">CrossRef
4. Cantell CE (1975) Anatomy, taxonomy, and biology of some Scandinavian heteronemertines of the genera / Lineus, / Micrura, and / Cerebratulus. Sarsia 58:89鈥?22
5. Chen H, Strand M, Norenburg JL, Sun S, Kajihara H, Chernyshev AV, Maslakova SA, Sundberg P (2010) Statistical parsimony networks and species assemblages in Cephalotrichid nemerteans (Nemertea). PLoS ONE 5(9):e12885. doi:10.1371/journal.pone.0012885 class="external" href="http://dx.doi.org/10.1371/journal.pone.0012885" target="_blank" title="It opens in new window">CrossRef
6. Coe WR (1945) / Malacobdella minuta, a new commensal nemertean. J Wash Acad Sci 35:65鈥?7
7. de Blainville HM (1827) Sangsue. In: Levrault EG (ed) Dictionnaire des Sciences Naturelles, 47. Normant, Paris, pp 270鈥?71
8. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783鈥?91 class="external" href="http://dx.doi.org/10.2307/2408678" target="_blank" title="It opens in new window">CrossRef
9. Fern谩ndez-脕lvarez F脕, Machordom A (2013) DNA barcoding reveals a cryptic nemertean invasion in Atlantic and Mediterranean waters. Helgoland Mar Res 67(3):599鈥?05 class="external" href="http://dx.doi.org/10.1007/s10152-013-0346-3" target="_blank" title="It opens in new window">CrossRef
10. Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome / c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotech 3:294鈥?99
11. Gibson R (1967) Occurrence of the entocommensal rhynchocoelan, / Malacobdella grossa, in the oval piddock, / Zirfaea crispata, on the Yorkshire coast. J Mar Biol Assoc UK 49:301鈥?17 class="external" href="http://dx.doi.org/10.1017/S0025315400056411" target="_blank" title="It opens in new window">CrossRef
12. Gibson R (1968) Studies on the biology of the entocommensal rhynchocoelan / Malacobdella grossa. J Mar Biol Assoc UK 48:637鈥?56 class="external" href="http://dx.doi.org/10.1017/S0025315400019202" target="_blank" title="It opens in new window">CrossRef
13. Gibson R (1994) Nemerteans. Synopses of the British fauna (new series, no. 24), 2nd edn. The Dorset Press, Dorchester, UK
14. Gibson R (1995) Nemertean genera and species of the world: an annotated checklist of original names and description citations, synonyms, current taxonomic status, habitats and recorded zoogeographic distribution. J Nat Hist 29:1鈥?62 class="external" href="http://dx.doi.org/10.1080/00222939500770161" target="_blank" title="It opens in new window">CrossRef
15. Gibson R, Jennings JB (1969) Observations on the diet, feeding mechanisms, digestion and food reserves of the entocommensal rhynchocoelan / Malacobdella grossa. J Mar Biol Assoc UK 49:17鈥?2 class="external" href="http://dx.doi.org/10.1017/S0025315400046397" target="_blank" title="It opens in new window">CrossRef
16. Huelsenbeck JP (2000) MrBayes: Bayesian inference of phylogeny. University of Rochester, Rochester, NY, Department of Biology
17. Huelsenbeck JP, Ronquist F (2001) MrBayes: Bayesian inference of phylogeny. Bioinformatics 17:754鈥?55 class="external" href="http://dx.doi.org/10.1093/bioinformatics/17.8.754" target="_blank" title="It opens in new window">CrossRef
18. Ivanov VA, Bigatti G, Penchaszadeh PE, Norenburg JL (2002) / Malacobdella arrokeana (Nemertea: Bdellonemertea), a new species of nemertean from the southwestern Atlantic Ocean entocommensal in / Panopea abbreviata (Bivalvia, Heterodonta, Hiatellidae) in Argentina. P Biol Soc Wash 115:359鈥?67
19. Jensen K, Sadeghian PS (2005) Nemertea. In: Rohde K (ed) Marine parasitology. CSIRO press, Australia, pp 205鈥?10
20. Junoy J, Andrade SCS, Giribet G (2010) Phylogenetic placement of a new hoplonemertean species commensal on ascidians. Invertebr Syst 24:616鈥?29 class="external" href="http://dx.doi.org/10.1071/IS10036" target="_blank" title="It opens in new window">CrossRef
21. Kajihara H, Olympia M, Kobayashi N, Katoh T, Chen HX, Strand M, Sundberg P (2011) Systematics and phylogeny of the hoplonemertean genus / Diplomma (Nemertea) based on molecular and morphological evidence. Zool J Linn Soc 161:695鈥?22 class="external" href="http://dx.doi.org/10.1111/j.1096-3642.2010.00650.x" target="_blank" title="It opens in new window">CrossRef
22. Kozloff EN (1991) / Malacobdella siliquae sp. nov. and / Malacobdella macomae sp. nov., commensal nemerteans from bivalve mollusks on the Pacific coast of North America. Can J Zool 66:1612鈥?618 class="external" href="http://dx.doi.org/10.1139/z91-225" target="_blank" title="It opens in new window">CrossRef
23. Kvist S, Laumer C, Junoy J, Giribet G (2013) New insights into the phylogeny, systematics and DNA barcoding of Nemertea. Invertebr Syst 28:287鈥?08 class="external" href="http://dx.doi.org/10.1071/IS13061" target="_blank" title="It opens in new window">CrossRef
24. Linnaeus C (1758) Systema naturae per regna tria naturae: secundum classes, ordines, genera, species, cum characteribus, differentiis, synonymis, locis, vol I, 10th edn. Laurentius Salvius, Stockholm, pp 824
25. Linnaeus C (1767) Systema naturae per regna tria naturae, secundum classes, ordines, genera, species, cum characteribus & differentiis. Vol. II. Salvius, Holmiae, p 735
26. Machordom A, Araujo R, Erpenbeck D, Ramos MA (2003) Phylogeography and conservation genetics of endangered European Margaritiferidae (Bivalvia: Unionoidea). Biol J Linn Soc 78:235鈥?52 class="external" href="http://dx.doi.org/10.1046/j.1095-8312.2003.00158.x" target="_blank" title="It opens in new window">CrossRef
27. Maslakova SA, von D枚hren J (2009) Larval development with transitory epidermis in / Paranemertes peregrina and other hoplonemerteans. Biol Bull 216:273鈥?92
28. Mateos E, Giribet G (2008) Exploring the molecular diversity of terrestrial nemerteans (Hoplonemertea, Monostilifera, Acteonemertidae) in a continental landmass. Zool Scr 37:235鈥?43 class="external" href="http://dx.doi.org/10.1111/j.1463-6409.2008.00324.x" target="_blank" title="It opens in new window">CrossRef
29. M眉ller OF (1776) Zoologiae Danicae prodromus, seu Animalium Daniae et Norvegiae indigenarum characters, nomina, et synonyma imprimis popularium. Typis Hallageriis, Havniae, Copenhagen
30. Posada D (2003) Using Modeltest and PAUP* to select a model of nucleotide substitution. In: Baxevanis AD, Davison DB, Page RDM, Petsko GA, Stein LD, Stormo GD (eds) Current protocols in bioinformatics. John Wiley & Sons, New York, pp 6.5.1鈥?.5.14
31. Posada D (2008) jModelTest: phylogenetic model averaging. Mol Biol Evol 25:1253鈥?256 class="external" href="http://dx.doi.org/10.1093/molbev/msn083" target="_blank" title="It opens in new window">CrossRef
32. Puerta P, Andrade SCS, Junoy J (2010) Redescription of / Lineus acutifrons Southern, 1913 (Nemertea) and comments on its phylogenetic position. J Nat Hist 44:37鈥?0 class="external" href="http://dx.doi.org/10.1080/00222933.2010.504895" target="_blank" title="It opens in new window">CrossRef
33. Riepen O (1933) Anatomie und histologic von / Malacobdella grossa (M眉ll.). Z Wiss Zool Abt A 143:323鈥?96
34. Strand M, Sundberg P (2005) Delimiting species in the hoplonemertean genus / Tetrastemma (phylum Nemertea): morphology is not concordant with phylogeny as evidenced from mtDNA sequences. Biol J Linn Soc 86:201鈥?12 class="external" href="http://dx.doi.org/10.1111/j.1095-8312.2005.00535.x" target="_blank" title="It opens in new window">CrossRef
35. Strand M, Hererra-Bachiller A, Nygren A, K氓nneby T (2014) A new nemertean species: what are the useful characters for ribbon worm descriptions? J Mar Biol Assoc UK 94:317鈥?30 class="external" href="http://dx.doi.org/10.1017/S002531541300146X" target="_blank" title="It opens in new window">CrossRef
36. Sundberg P, Strand M (2010) Nemertean taxonomy - time to change lane? J Zool Syst Evol Res 48:283鈥?84
37. Sundberg P, Thur贸czy Vodoti E, Zhou H, Strand M (2009) Polymorphism hides cryptic species in / Oerstedia dorsalis (Nemertea, Hoplonemertea). Biol J Linn Soc 98:556鈥?67 class="external" href="http://dx.doi.org/10.1111/j.1095-8312.2009.01310.x" target="_blank" title="It opens in new window">CrossRef
38. Sundberg P, Vodoti E, Strand M (2010) DNA barcoding should accompany taxonomy the case of / Cerebratulus spp. (Nemertea). Mol Ecol Res 10:274鈥?81 class="external" href="http://dx.doi.org/10.1111/j.1755-0998.2009.02774.x" target="_blank" title="It opens in new window">CrossRef
39. Swofford DL (2000) / PAUP*. Phylogenetic analysis using parsimony (*and other methods), Version 4. Sinauer Associates, Sunderland, Massachusetts, USA
40. Taboada S, Junoy J, Andrade SCS, Giribet G, Cristobo J, Avila C (2013) On the identity of two Antarctic brooding nemerteans: redescription of / Antarctonemertes valida (Burger, 1893) and description of a new species in the genus / Antarctonemertes Friedrich, 1955 (Nemertea, Hoplonemertea). Polar Biol 36:1415鈥?430 class="external" href="http://dx.doi.org/10.1007/s00300-013-1360-2" target="_blank" title="It opens in new window">CrossRef
41. Takakura U (1897) On a new species of / Malacobdella ( / M. japonica). Annot Zool Japon 1:105鈥?12
42. Teso SV, Bigatti G, Bazterrica MC, Ciocco NF, Penchaszadeh PE (2006) The reproductive cycle of the entocommensal nemertean / Malacobdella arrokeana and its symbiosis with the geoduck / Panopea abbreviata. Invertebr Biol 125(4):314鈥?24 class="external" href="http://dx.doi.org/10.1111/j.1744-7410.2006.00063.x" target="_blank" title="It opens in new window">CrossRef
43. Thollesson M, Norenburg JL (2003) Ribbon worm relationships: a phylogeny of the phylum Nemertea. P Roy Soc Lond B Biol 270:407鈥?15 class="external" href="http://dx.doi.org/10.1098/rspb.2002.2254" target="_blank" title="It opens in new window">CrossRef
44. Thornhill DJ, Mahon AR, Norenburg JL, Halanych KM (2008) Open-ocean barriers to dispersal: a test case with the Antarctic Polar Front and the ribbon worm / Parborlasia corrugatus (Nemertea: Lineidae). Mol Ecol 17:5104鈥?117 class="external" href="http://dx.doi.org/10.1111/j.1365-294X.2008.03970.x" target="_blank" title="It opens in new window">CrossRef
45. Wiens JJ (2007) Species delimitation: new approaches for discovering diversity. Syst Biol 56:875鈥?78 class="external" href="http://dx.doi.org/10.1080/10635150701748506" target="_blank" title="It opens in new window">CrossRef
46. Yamaoka T (1940) The fauna of Akkeshi Bay. IX. Nemertini. J Fac Sci, U Hokkaido 6(7):205鈥?61 - 刊物主题：Zoology;
- 出版者：Springer Berlin Heidelberg
- ISSN：1810-522X
文摘
Background Nemerteans of the genus Malacobdella live inside of the mantle cavity of marine bivalves. The genus currently contains only six species, five of which are host-specific and usually found in a single host species, while the sixth species, M. grossa, has a wide host range and has been found in 27 different bivalve species to date. The main challenge of Malacobdella species identification resides in the similarity of the external morphology between species (terminal sucker, gut undulations number, anus position and gonad colouration), and thus, the illustrations provided in the original descriptions do not allow reliable identification. In this article, we analyse the relationships among three species of Malacobdella: M. arrokeana, M. japonica and M. grossa, adding new data for the M. grossa and reporting the first for M. japonica, analysing 658 base pairs of the mitochondrial cytochrome c oxidase subunit I gene (COI). Based on these analyses, we present and discuss the potential of DNA barcoding for Malacobdella species identification. Results Sixty-four DNA barcoding fragments of the mitochondrial COI gene from three different Malacobdella species (M. arrokeana, M. japonica and M. grossa) are analysed (24 of them newly sequenced for this study, along with four outgroup specimens) and used to delineate species. Divergences, measured as uncorrected differences, between the three species were M. arrokeana-M. grossa 11.73%, M. arrokeana-M. japonica 10.62% and M. grossa-M. japonica 10.97%. The mean intraspecific divergence within the ingroup species showed a patent gap with respect to the interspecific ones: 0.18% for M. arrokeana, 0.13% for M. grossa and 0.02% for M. japonica (ranges from 0 to 0.91%). Conclusions We conclude that there is a clear correspondence between the molecular data and distinguishing morphological characters. Our results thus indicate that some morphological characters are useful for species identification and support the potential of DNA barcoding for species identification in a taxonomic group with subtle morphological external differences.